Construct: ORF TRCN0000471036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015452.1_s317c1
- Derived from:
- ccsbBroadEn_10802
- DNA Barcode:
- TAGAGCCGATTGATCGTACCCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPB41L2 (2037)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471036
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001135554.1 | 92.7% | 91.1% | (many diffs) |
| 2 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001135555.3 | 92.7% | 91.1% | (many diffs) |
| 3 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350314.1 | 88.8% | 86.9% | (many diffs) |
| 4 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350315.1 | 88.8% | 86.9% | (many diffs) |
| 5 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001252660.1 | 82.8% | 82.5% | (many diffs) |
| 6 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350311.1 | 82.8% | 82.5% | (many diffs) |
| 7 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350312.1 | 82.8% | 82.5% | (many diffs) |
| 8 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350313.1 | 82.8% | 82.5% | (many diffs) |
| 9 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001199388.2 | 74.2% | 73.3% | 1870delA;1901_2556del |
| 10 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350308.1 | 74.2% | 73.3% | 1870delA;1901_2556del |
| 11 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350309.1 | 74.2% | 73.3% | 1870delA;1901_2556del |
| 12 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350310.1 | 74.2% | 73.3% | 1870delA;1901_2556del |
| 13 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350307.1 | 72.7% | 71.8% | 1831_1884del;1924delA;1955_2610del |
| 14 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350304.1 | 67.7% | 66.8% | 1870delA;1901_2805del |
| 15 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350305.1 | 67.7% | 66.8% | 1870delA;1901_2805del |
| 16 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350306.1 | 67.7% | 66.8% | 1870delA;1901_2805del |
| 17 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_011535528.2 | 66.4% | 65.5% | 1831_1884del;1924delA;1955_2859del |
| 18 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350303.1 | 66.2% | 65.3% | 1832_1894del;1933delA;1964_2868del |
| 19 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010356.2 | 64.9% | 63.4% | 1831_2196del;2236delA;2267_2922del |
| 20 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350302.1 | 62.9% | 62.1% | 1833_2042del;2080delA;2111_3015del |
| 21 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001431.4 | 62.9% | 62.1% | 1833_2042del;2080delA;2111_3015del |
| 22 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_011535527.2 | 61.8% | 61% | 1831_2094del;2134delA;2165_3069del |
| 23 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350301.1 | 61.8% | 60.3% | 1831_2196del;2236delA;2267_3072del |
| 24 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_011535525.2 | 60.9% | 60.1% | 1831_2142del;2182delA;2213_3117del |
| 25 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350299.1 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 26 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_011535524.2 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 27 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010350.2 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 28 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010351.1 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 29 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010352.1 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 30 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010353.2 | 59.8% | 58.4% | 1831_2196del;2236delA;2267_3171del |
| 31 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_024446349.1 | 58.6% | 57.2% | 1831_2262del;2302delA;2333_3237del |
| 32 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XR_001743217.2 | 45.2% | (many diffs) | |
| 33 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XR_001743216.2 | 42.6% | (many diffs) | |
| 34 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XR_001743213.2 | 39.2% | (many diffs) | |
| 35 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NR_146620.1 | 39.1% | (many diffs) | |
| 36 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XR_001743215.2 | 28.1% | (many diffs) | |
| 37 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | NM_001350320.1 | 24.7% | 23.8% | 0_1ins1206;664delA;695_1599del |
| 38 | human | 2037 | EPB41L2 | erythrocyte membrane protei... | XM_017010364.1 | 21.8% | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1965
- ORF length:
- 1899
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tactgaagta ggctctgtgt ctgaagtgaa gaaggactct agccagttag 121 gaacagatgc aaccaaggaa aaacctaaag aagtagcaga aaatcagcag aatcagtctt 181 ccgatccaga ggaggaaaaa ggttcccagc cacctcctgc agctgaaagc caaagtagtc 241 tacgccgcca gaagagagag aaggaaacat cggagagcag gggtatttct cggttcatac 301 cgccatggct taagaagcaa aagtcatata ccttagtagt ggccaaagat ggaggagata 361 aaaaagagcc tacccaagct gttgttgaag aacaggtctt agataaagag gaaccccttc 421 cagaagaaca gagacaggct aagggtgatg ctgaagaaat ggctcagaag aaacaagaga 481 ttaaagttga agtcaaggaa gaaaaaccct cagtgagcaa ggaagaaaaa ccctcagtga 541 gcaaagtgga gatgcagcct actgaattag taagtaagga gagagaagag aaggtaaaag 601 aaacacagga agacaaatta gaaggaggag cagcaaaaag ggagaccaag gaagtgcaga 661 ccaatgagct gaaagcagag aaggcatctc aaaaagtcac caagaagacc aaaactgtcc 721 agtgtaaagt gaccctctta gatggcaccg aatacagctg tgacctggag aaacatgcca 781 agggacaagt gttatttgac aaagtgtgtg aacacctcaa tctcttggag aaagactact 841 ttggactttt gtttcaggaa agccctgagc agaaaaactg gttagatcct gctaaagaaa 901 taaagagaca actgagaaac cttccatggc tattcacttt taatgtgaag ttttatcctc 961 ctgatccttc tcaattgact gaagatatca ccagatactt cttgtgcctt cagctccggc 1021 aggacattgc ctctggccgc ctgccctgct cttttgtgac tcatgctctc ctgggatcct 1081 acaccctgca ggctgaactt ggtgactatg acccagaaga acatggcagc atcgacctca 1141 gtgaattcca gtttgcccct actcagacta aggagctgga agagaaggtg gcagagctgc 1201 acaaaaccca caggggctta tcgccagcac aagctgattc ccagttctta gaaaatgcaa 1261 agaggctttc catgtatggt gttgacctac atcatgccaa ggactcagaa ggtgtggaca 1321 tcaagctggg cgtgtgtgct aatggacttc tcatttacaa agacagactg cgaatcaatc 1381 gttttgcttg gccgaaaatc ttaaaaattt cctataaacg cagtaacttc tacattaaag 1441 tcagaccggc agagctggaa cagtttgaga gtaccattGG ATTCAAACTG CCAAACCACC 1501 GGGCAGCGAA AAGACTATGG AAAGTGTGCG TGGAGCATCA TACTTTCTAC AGGCTTGTTT 1561 CTCCAGAGCA GCCACCAAAA GCCAAGTTCC TGACCTTGGG GTCCAAATTT CGCTATAGTG 1621 GCCGCACCCA AGCACAGACC CGCCAGGCCA GCACCCTCAT AGATAGGCCA GCACCACACT 1681 TTGAGCGCAC TTCTAGTAAA CGGGTCTCCA GGAGTCTAGA TGGAGCTCCG ATTGGTGTCA 1741 TGGACCAAAG TCTTATGAAG GATTTTCCTG GCGCTGCTGG GGAGATTTCA GCCTATGGAC 1801 CTGGACTTGT CAGCATTGCC GTGGTACAAG ATGGGGACGG CAGGAGGGAA GTGAGAAGCC 1861 CAACTAAAGC CCCACATTTG CAGCTCATTG AAGGAAAGAG TTCACATGAG ACTCTGAATA 1921 TAGTGGAGGA GAAGAGCGGG CAGAGGTTGG GAAAGACGAA AGAGTACCCA ACTTTCTTGT 1981 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2041 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2101 GAAAGGACGA TAGAGCCGAT TGATCGTACC CGCCACGCGT TAAGTCgaca atcaacctct 2161 ggattacaaa atttgtgaaa gatt