Construct: ORF TRCN0000471044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004281.1_s317c1
- Derived from:
- ccsbBroadEn_05664
- DNA Barcode:
- TCTTTCGACTCATCCGAGCTAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX19 (415117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 415117 | STX19 | syntaxin 19 | NM_001001850.3 | 99.8% | 100% | 744C>T |
| 2 | human | 415117 | STX19 | syntaxin 19 | XM_011512842.2 | 99.8% | 100% | 744C>T |
| 3 | mouse | 68159 | Stx19 | syntaxin 19 | NM_026588.1 | 85% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agaccgactt caagaactaa agcagagaac aaaggaaatt gaactctcta 121 gagacagtca tgtatcaact acagaaacag aggaacaagg ggtgtttcta cagcaagctg 181 ttatttatga aagagagcct gtagctgaga gacacctaca tgaaatccaa aaactacagg 241 aaagtattaa caatttggca gataatgttc aaaaatttgg gcagcaacag aaaagtctgg 301 tggcttcaat gagaaggttt agtctactta agagagagtc taccattaca aaggagataa 361 aaattcaggc agaatacatc aacagaagtt tgaatgattt agttaaagaa gttaaaaagt 421 cagaggttga aaatggtcca tcttcagtgg tcacaaggat acttaaatct cagcatgctg 481 caatgttccg ccattttcag caaatcatgt ttatatacaa tgacacaata gcagcaaagc 541 aagagaagtg caagacattt attttacgtc agcttgaagt tgctggaaaa gagatgtctg 601 aagaagatgt aaaTGATATG CTTCATCAAG GAAAATGGGA AGTTTTTAAT GAAAGCTTAC 661 TTACAGAAAT CAATATCACT AAAGCACAAC TTTCAGAGAT TGAACAGAGA CACAAGGAAC 721 TTGTTAATTT GGAGAACCAA ATAAAGGATT TAAGGGATCT TTTCATTCAG ATATCTCTTT 781 TAGTAGAGGA ACAAGGAGAG AGCATCAATA ATATTGAAAT GACAGTGAAT AGTACAAAAG 841 AGTATGTTAA CAATACTAAA GAGAAATTTG GACTAGCTGT AAAATACAAA AAAAGAAATC 901 CTTGCAGAGT ACTGTGTTGT TGGTGCTGTC CATGCTGTAG CTCAAAATAC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGATCTTTCG ACTCATCCGA GCTAAGAACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt