Construct: ORF TRCN0000471046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012591.1_s317c1
- Derived from:
- ccsbBroadEn_13927
- DNA Barcode:
- TGCGGCTCATGCCCGGTGATCATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP3R1 (5534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5534 | PPP3R1 | protein phosphatase 3 regul... | NM_000945.4 | 99.6% | 2.3% | 6_7delAAinsC |
| 2 | mouse | 19058 | Ppp3r1 | protein phosphatase 3, regu... | NM_024459.2 | 94.1% | 2.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 132
- ORF length:
- 66
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg catgaggcaa gttatccttt ggaaatgtgc tcacactttg atgcggatga 121 aattaaaagg ctaggaaaga gatttaagaa gcttgatttg gacaattctg gttctttgag 181 tgtggaagag ttcatgtctc tgcctgagtt acaaCAGAAT CCTTTAGTAC AGCGAGTAAT 241 AGATATATTC GACACAGATG GGAATGGAGA AGTAGACTTT AAAGAATTCA TTGAGGGCGT 301 CTCTCAGTTC AGTGTCAAAG GAGATAAGGA GCAGAAATTG AGGTTTGCTT TCCGTATCTA 361 TGACATGGAT AAAGATGGCT ATATTTCCAA TGGGGAACTC TTCCAGGTAT TGAAGATGAT 421 GGTGGGGAAC AATCTGAAAG ATACACAGTT ACAGCAAATT GTAGACAAAA CCATAATAAA 481 TGCAGATAAG GATGGAGATG GAAGAATATC CTTTGAAGAA TTCTGTGCTG TTGTAGGTGG 541 CCTAGATATC CACAAAAAGA TGGTGGTAGA TGTGTGCCCA ACTTTCTTGT ACAAAGTGGT 601 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 661 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 721 TGCGGCTCAT GCCCGGTGAT CATAACGCGT TAAGTCgaca atcaacctct ggattacaaa 781 atttgtgaaa gatt