Construct: ORF TRCN0000471059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000945.1_s317c1
- Derived from:
- ccsbBroadEn_11744
- DNA Barcode:
- AACAGATTTCATCTCAGGATACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK8IP2 (23542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23542 | MAPK8IP2 | mitogen-activated protein k... | XM_011530681.2 | 56.7% | 56.7% | 265_1275del |
| 2 | human | 23542 | MAPK8IP2 | mitogen-activated protein k... | XM_011530680.2 | 56.2% | 56.2% | 265_1296del |
| 3 | human | 23542 | MAPK8IP2 | mitogen-activated protein k... | NM_012324.6 | 53.7% | 53.7% | 265_1407del |
| 4 | human | 23542 | MAPK8IP2 | mitogen-activated protein k... | XM_011530679.2 | 53.6% | 53.6% | 265_1410del |
| 5 | mouse | 60597 | Mapk8ip2 | mitogen-activated protein k... | NM_021921.3 | 47.4% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatcgcgcg gagatgtttt ctctctccac cttccactcg ctgtcgccgc 121 cgggctgcag gcctccccag gacataagcc tggaagaatt tgacgacgaa gatctgtctg 181 agatcactga tgactgtggc ctgggcctca gctacgactc agaccactgt gagaaggaca 241 gcctctccct ggggcgctcg gagcagccgc accccatctg ctccttccag gatgacttcc 301 aggagtttga gatgatcgat gacaatgaag aggaggacga agaggacgac gaggaagagg 361 aggatgccga ggacagtgcg gggtcccccg ggggcagggg cacgggcccc tcggcgccgc 421 gggacgcgtc gctggtgtac gacgcggtca agtacacgct ggtggtggat gagcacacgc 481 agctggagct ggtgagcctg cggcgctgtg ctgggctggg ccacgacagc gaagaggaca 541 gcggcgggga ggccagcgag gaggaggcgg gcgcggcgct gctaggcggc ggtcaggtct 601 cgggggacac ctcgccggac agccctgacc tcactttctc caagaagttc ctcaatgtct 661 tcgtcaacag cacatctcgg tcctccagca ccgagtcctt tggccttttc tcctgtctgg 721 tcaacggcga ggagcgagag cagactcacc gggctgtgtt caggttcatc ccgcggcatc 781 cagacgagct ggagctggat gtggatgacc ctgtgttggt ggaggccgag gaggacgact 841 tctggttccg tggcttcaac atgcgcacgg gggagcgcgg tgtgtttcct gccttctacg 901 cccatgcggt gcccggccct gccaaggacc tgctggggag taagcggagc ccctgctggg 961 tggagcgctt tgacgtgcag ttcctgggct ccgtggaggt gccctgccac cagggcaacg 1021 gcatcctgtg tgcagcCATG CAGAAGATTG CCACTGCCCG GAAACTGACC GTCCACCTGC 1081 GCCCTCCTGC CTCCTGTGAC CTCGAGATCT CTCTTCGGGG GGTCAAGCTG AGTCTGAGCG 1141 GAGGAGGGCC CGAGTTCCAG CGCTGCAGCC ATTTCTTCCA GATGAAGAAC ATCTCCTTCT 1201 GCGGCTGCCA TCCCCGCAAC AGCTGCTATT TCGGCTTCAT CACCAAACAC CCCCTGCTGA 1261 GCCGCTTCGC CTGCCACGTC TTTGTCTCCC AGGAGTCCAT GAGGCCGGTG GCGCAGAGTG 1321 TGGGCCGCGC CTTCCTGGAG TACTACCAAG AGCACCTGGC GTACGCCTGC CCCACGGAGG 1381 ACATCTACCT GGAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACAGATTTC ATCTCAGGAT 1561 ACAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt