Construct: ORF TRCN0000471062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000903.1_s317c1
- Derived from:
- BRDN0000390012
- DNA Barcode:
- AGCTATAACGCCGGTCCCCTTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG9A (79065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79065 | ATG9A | autophagy related 9A | NM_001077198.3 | 62.6% | 52.9% | (many diffs) |
| 2 | human | 79065 | ATG9A | autophagy related 9A | NM_024085.5 | 62.6% | 52.9% | (many diffs) |
| 3 | human | 79065 | ATG9A | autophagy related 9A | NR_104255.2 | 42.4% | (many diffs) | |
| 4 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238690.2 | 61.3% | 55.6% | (many diffs) |
| 5 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238689.2 | 58.7% | 53.3% | (many diffs) |
| 6 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001003917.4 | 58% | 52.7% | (many diffs) |
| 7 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001288612.1 | 58% | 52.7% | (many diffs) |
| 8 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238687.2 | 58% | 52.7% | (many diffs) |
| 9 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238688.2 | 58% | 52.7% | (many diffs) |
| 10 | mouse | 245860 | Atg9a | autophagy related 9A | NR_109938.1 | 38.4% | (many diffs) | |
| 11 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001288613.1 | 34% | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagtttgac actgctggcc agcgcctaga ggcctcctat agtgattcac 121 ccccagggga ggaggacctg ttggtgcacg tcgccgaggg gagcaagtca ccttggcacc 181 atattgaaaa ccttgacctc ttcttctctc gagtttataa tctgcaccag aagaatggct 241 tcacatgtat gctcatcggg gagatctttg agctcatgca gttcctcttt gtggttgcct 301 tcactacctt cctggtcagc tgcgtggact atgacatcct atttgccaac aagatggtga 361 accacagtct tcaccctact gaacccgtca aggtcactct gccagacgcc tttttgcctg 421 ctcaagtctg tagtgccagg attcaggaaa atggctccct tatcaccatc ctggtcattg 481 ctggtgtctt ctggatccac cggcttatca agttcatcta taacatttgc tgctactggg 541 agatccactc cttctacctg cacgctctgc gcatccctat gtctgccctt ccgtattgca 601 cgtggcaaga agtgcaggcc cggatcgtgc agacgcagaa ggagcaccag atctgcatcc 661 acaaacgtga gctgacagaa ctggacatct accaccgcat cctccgtttc cagaactaca 721 tggtggcact ggttaacaaa tccctcctgc ctctgcgctt ccgcctgcct ggcctcgggg 781 aagctgtctt cttcacccgt ggtctcaagt acaactttga gctgatcctc ttctggggac 841 ctggctctct gtttctcaat gaatggagcc tcaaggccga gtacaaacgt ggggggcaac 901 ggctagagct ggcccagcgc ctcagcaacc gcatcctgtg gattggcatc gctaacttcc 961 tgctgtgccc cctcatcctc atatggcaaa tcctctatgc cttcttcagc tatgctgagg 1021 tgctgaagcg ggagccgggg gccctgggag cacgctgctg gtcactctat ggccgctgct 1081 acctccgcca cttcaacgag ctggagcacg agctgcagtc ccgcctcaac cgtggctaca 1141 agcccgcctc caagtacatg aattgcttct tgtcacctag tttgacactg ctggccaaga 1201 atggaacctt cttcgctggc tccatcctgg ctgtgcttat tgccctcacc atttatgacg 1261 aagatgtgtt GGCTGTGGAA CATGTGCTGA CCACCGTCAC ACTCCTGGGG GTCACCGTGA 1321 CCGTGTGCAG GTCCTTTATC CCGGACCAGC ACATGGTGTT CTGCCCTGAG CAGCTGCTCC 1381 GCGTGATCCT CGCTCACATC CACTACATGC CTGACCACTG GCAGGGTGTT CATTTTGGAA 1441 GAGTTGCTGA GCCCCATTGT CACACCCCTC ATCCTCATCT TCTGCCTGCG CCCACGGGCC 1501 CTGGAGATTA TAGACTTCTT CCGAAACTTC ACCGTGGAGG TCGTTGGTGT GGGAGATACC 1561 TGCTCCTTTG CTCAGATGGA TGTTCGCCAG CATGGTCATC CCCAGTGGCT ATCTGCTGGG 1621 CAGACAGAGG CCTCAGTGTA CCAGCAAGCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCTA 1801 TAACGCCGGT CCCCTTAACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt