Construct: ORF TRCN0000471072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017646.1_s317c1
- Derived from:
- ccsbBroadEn_00113
- DNA Barcode:
- TGCTAAAATGAAGAACCGACTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASNS (440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001352496.2 | 100% | 100% | |
| 2 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001673.5 | 100% | 100% | |
| 3 | human | 440 | ASNS | asparagine synthetase (glut... | NM_133436.3 | 100% | 100% | |
| 4 | human | 440 | ASNS | asparagine synthetase (glut... | NM_183356.3 | 100% | 100% | |
| 5 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178075.2 | 96.2% | 96.2% | 0_1ins63 |
| 6 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178076.2 | 85.2% | 85.2% | 0_1ins249 |
| 7 | human | 440 | ASNS | asparagine synthetase (glut... | NM_001178077.1 | 85.2% | 85.2% | 0_1ins249 |
| 8 | human | 389652 | ASNSP1 | asparagine synthetase pseud... | NR_146077.1 | 51.9% | (many diffs) | |
| 9 | human | 107986827 | CZ1P-ASNS | CZ1P-ASNS readthrough | NR_147989.1 | 47.6% | 1_1629del;2765_2838del;3387_3531del | |
| 10 | mouse | 27053 | Asns | asparagine synthetase | NM_012055.3 | 87.7% | 93.9% | (many diffs) |
| 11 | mouse | 27053 | Asns | asparagine synthetase | XM_006505092.3 | 87.7% | 93.9% | (many diffs) |
| 12 | mouse | 27053 | Asns | asparagine synthetase | XM_006505093.3 | 87.7% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1749
- ORF length:
- 1683
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tggcatttgg gcgctgtttg gcagtgatga ttgcctttct gttcagtgtc 121 tgagtgctat gaagattgca cacagaggtc cagatgcatt ccgttttgag aatgtcaatg 181 gatacaccaa ctgctgcttt ggatttcacc ggttggcggt agttgacccg ctgtttggaa 241 tgcagccaat tcgagtgaag aaatatccgt atttgtggct ctgttacaat ggtgaaatct 301 acaaccataa gaagatgcaa cagcattttg aatttgaata ccagaccaaa gtggatggtg 361 agataatcct tcatctttat gacaaaggag gaattgagca aacaatttgt atgttggatg 421 gtgtgtttgc atttgtttta ctggatactg ccaataagaa agtgttcctg ggtagagata 481 catatggagt cagacctttg tttaaagcaa tgacagaaga tggatttttg gctgtatgtt 541 cagaagctaa aggtcttgtt acattgaagc actccgcgac tcccttttta aaagtggagc 601 cttttcttcc tggacactat gaagttttgg atttaaagcc aaatggcaaa gttgcatccg 661 tggaaatggt taaatatcat cactgtcggg atgtacccct gcacgccctc tatgacaatg 721 tggagaaact ctttccaggt tttgagatag aaactgtgaa gaacaacctc aggatccttt 781 ttaataatgc tgtaaagaaa cgtttgatga cagacagaag gattggctgc cttttatcag 841 ggggcttgga ctccagcttg gttgctgcca ctctgttgaa gcagctgaaa gaagcccaag 901 tacagtatcc tctccagaca tttgcaattg gcatggaaga cagccccgat ttactggctg 961 ctagaaaggt ggcagatcat attggaagtg aacattatga agtccttttt aactctgagg 1021 aaggcattca ggctctggat gaagtcatat tttccttgga aacttatgac attacaacag 1081 ttcgtgcttc agtaggtatg tatttaattt ccaagtatat tcggaagaac acagatagcg 1141 tggtgatctt ctctggagaa ggatcagatg aacttacgca gggttacata tattttcaca 1201 aggctccttc tcctGAAAAA GCCGAGGAGG AGAGTGAGAG GCTTCTGAGG GAACTCTATT 1261 TGTTTGATGT TCTCCGCGCA GATCGAACTA CTGCTGCCCA TGGTCTTGAA CTGAGAGTCC 1321 CATTTCTAGA TCATCGATTT TCTTCCTATT ACTTGTCTCT GCCACCAGAA ATGAGAATTC 1381 CAAAGAATGG GATAGAAAAA CATCTCCTGA GAGAGACGTT TGAGGATTCC AATCTGATAC 1441 CCAAAGAGAT TCTCTGGCGA CCAAAAGAAG CCTTCAGTGA TGGAATAACT TCAGTTAAGA 1501 ATTCCTGGTT TAAGATTTTA CAGGAATACG TTGAACATCA GGTTGATGAT GCAATGATGG 1561 CAAATGCAGC CCAGAAATTT CCCTTCAATA CTCCTAAAAC CAAAGAAGGA TATTACTACC 1621 GTCAAGTCTT TGAACGCCAT TACCCAGGCC GGGCTGACTG GCTGAGCCAT TACTGGATGC 1681 CCAAGTGGAT CAATGCCACT GACCCTTCTG CCCGCACGCT GACCCACTAC AAGTCAGCTG 1741 TCAAAGCTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1801 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1861 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGCTAA AATGAAGAAC CGACTTTTAC 1921 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt