Transcript: Mouse NM_012055.3

Mus musculus asparagine synthetase (Asns), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Asns (27053)
Length:
1927
CDS:
98..1783

Additional Resources:

NCBI RefSeq record:
NM_012055.3
NBCI Gene record:
Asns (27053)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_012055.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000031699 CGTGAAGAACAATCTGCGTAT pLKO.1 787 CDS 100% 4.050 5.670 N Asns n/a
2 TRCN0000324779 CGTGAAGAACAATCTGCGTAT pLKO_005 787 CDS 100% 4.050 5.670 N Asns n/a
3 TRCN0000031702 CCCAGAAGTTTCCCTTCAATA pLKO.1 1602 CDS 100% 13.200 9.240 N Asns n/a
4 TRCN0000324781 CCCAGAAGTTTCCCTTCAATA pLKO_005 1602 CDS 100% 13.200 9.240 N Asns n/a
5 TRCN0000045873 GCTCTGTTACAATGGTGAAAT pLKO.1 310 CDS 100% 13.200 9.240 N ASNS n/a
6 TRCN0000031701 CCCTTATTTGTGGCTCTGTTA pLKO.1 298 CDS 100% 4.950 3.465 N Asns n/a
7 TRCN0000324780 CCCTTATTTGTGGCTCTGTTA pLKO_005 298 CDS 100% 4.950 3.465 N Asns n/a
8 TRCN0000031700 GCCAGATATGAGAATTCCAAA pLKO.1 1396 CDS 100% 0.000 0.000 N Asns n/a
9 TRCN0000324782 GCCAGATATGAGAATTCCAAA pLKO_005 1396 CDS 100% 0.000 0.000 N Asns n/a
10 TRCN0000031703 CGCTATCAAGAAACGCTTGAT pLKO.1 820 CDS 100% 0.495 0.297 N Asns n/a
11 TRCN0000324928 CGCTATCAAGAAACGCTTGAT pLKO_005 820 CDS 100% 0.495 0.297 N Asns n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_012055.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05860 pDONR223 100% 87.8% 94.1% None (many diffs) n/a
2 ccsbBroad304_05860 pLX_304 0% 87.8% 94.1% V5 (many diffs) n/a
3 TRCN0000474976 TCCTTTCCATGGACTAAAGCAGGT pLX_317 32% 87.8% 94.1% V5 (many diffs) n/a
4 ccsbBroadEn_00113 pDONR223 100% 87.7% 93.9% None (many diffs) n/a
5 ccsbBroad304_00113 pLX_304 0% 87.7% 93.9% V5 (many diffs) n/a
6 TRCN0000471072 TGCTAAAATGAAGAACCGACTTTT pLX_317 32% 87.7% 93.9% V5 (many diffs) n/a
Download CSV