Construct: ORF TRCN0000471089

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016284.1_s317c1
Derived from:
ccsbBroadEn_00647
DNA Barcode:
GATGCTGGGTGATTGAACTATGAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GLRA2 (2742)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471089
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2742 GLRA2 glycine receptor alpha 2 NM_001118885.1 100% 100%
2 human 2742 GLRA2 glycine receptor alpha 2 NM_002063.4 100% 100%
3 human 2742 GLRA2 glycine receptor alpha 2 NM_001118886.2 99% 99.5% (many diffs)
4 human 2742 GLRA2 glycine receptor alpha 2 XM_017029427.1 99% 99.5% (many diffs)
5 human 2742 GLRA2 glycine receptor alpha 2 XM_006724487.3 96.2% 95.1% (many diffs)
6 human 2742 GLRA2 glycine receptor alpha 2 XM_011545496.2 96.2% 95.1% (many diffs)
7 human 2742 GLRA2 glycine receptor alpha 2 XM_024452365.1 96.2% 95.1% (many diffs)
8 human 2742 GLRA2 glycine receptor alpha 2 NM_001171942.1 80.3% 80.3% 0_1ins267
9 human 2742 GLRA2 glycine receptor alpha 2 XM_017029428.2 80.3% 80.3% 0_1ins267
10 human 2742 GLRA2 glycine receptor alpha 2 XM_017029429.2 80.3% 80.3% 0_1ins267
11 human 2742 GLRA2 glycine receptor alpha 2 XM_024452366.1 80.3% 80.3% 0_1ins267
12 mouse 237213 Glra2 glycine receptor, alpha 2 s... NM_183427.4 92.8% 98.6% (many diffs)
13 mouse 237213 Glra2 glycine receptor, alpha 2 s... XM_006528834.1 92.8% 98.6% (many diffs)
14 mouse 237213 Glra2 glycine receptor, alpha 2 s... XM_006528835.3 92.8% 98.6% (many diffs)
15 mouse 237213 Glra2 glycine receptor, alpha 2 s... XM_006528833.1 91.9% 98.2% (many diffs)
16 mouse 237213 Glra2 glycine receptor, alpha 2 s... XM_006528836.3 82.3% 88.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1422
ORF length:
1356
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ccggcagcta gtgaacattt tgacagcctt gtttgcattt ttcttagaga
121caaaccactt caggacggct ttctgcaaag accatgactc caggtctgga aaacaacctt
181cacagaccct atctccttca gatttcttgg acaagttaat gggaaggaca tcaggatatg
241atgcaagaat caggccaaat tttaaaggtc ctccagtaaa cgttacttgc aatattttta
301tcaacagttt tggatcagtc acagaaacga ccatggacta ccgagtgaat atttttctga
361gacaacagtg gaatgattca cggctggcgt acagtgagta cccagatgac tccctggact
421tggacccatc catgctagac tccatttgga aaccagattt gttctttgcc aatgagaagg
481gtgccaactt ccacgatgtc accactgaca acaaattgct acggatttcg aaaaatggca
541aagtgctcta cagtatcaga ctcaccttga ccttatcctg tcccatggac ttgaagaact
601ttccgatgga tgtccagacc tgtacaatgc agctggagag ttttgggtac acgatgaatg
661acctgatatt tgagtggtta agtgatggtc cagtgcaagt tgctgaagga ttgaccctgc
721cccagtttat tttgaaagaa gagaaggaac ttggctactg tacaaagcac tacaacactg
781gaaagtttac ctgcattgag gtcaagtttc atctggaacg ccaaatggga tattatttga
841tccagatgta catcccaagc ctgcttatag taattttgtc ctgggtttcc ttttggataa
901atatggatgc agcccctgcc agggtcgcac tgggcatcac cacagtctta acgatgacca
961cccagagttc aggctccagg gcatctctgc caaaggtctc ctatgtaaaa gcgattgaca
1021tctggatggc ggtgtgcctt ctgtttgTGT TTGCTGCCTT ACTGGAATAC GCAGCGGTGA
1081ACTTCGTCTC CAGGCAACAC AAGGAGTTCC TGCGCCTCCG AAGAAGACAG AAGAGGCAGA
1141ATAAGGAAGA AGACGTTACT CGTGAAAGTC GTTTTAATTT TAGCGGTTAT GGGATGGGTC
1201ACTGCCTCCA AGTGAAAGAT GGAACAGCTG TCAAGGCCAC ACCTGCCAAC CCACTCCCAC
1261AACCGCCAAA AGATGGAGAT GCTATCAAGA AGAAGTTTGT GGACCGGGCA AAAAGGATTG
1321ACACGATATC TCGAGCTGCC TTCCCATTGG CCTTCCTCAT TTTCAACATC TTTTACTGGA
1381TCACATACAA GATCATTCGG CATGAAGATG TCCACAAGAA ATACCCAACT TTCTTGTACA
1441AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1501AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1561AGGACGAGAT GCTGGGTGAT TGAACTATGA GACGCGTTAA GTCgacaatc aacctctgga
1621ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)