Construct: ORF TRCN0000471113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019052.3_s317c1
- Derived from:
- ccsbBroadEn_14878
- DNA Barcode:
- TACTGTGATCAAACCCTAGACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YES1 (7525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471113
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | NM_005433.4 | 99.9% | 100% | 1444T>C |
| 2 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_017025960.2 | 99.9% | 100% | 1444T>C |
| 3 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451243.1 | 99.9% | 100% | 1444T>C |
| 4 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451244.1 | 99.9% | 100% | 1444T>C |
| 5 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451245.1 | 99.9% | 100% | 1444T>C |
| 6 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451246.1 | 99.9% | 100% | 1444T>C |
| 7 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_005258139.4 | 91.8% | 91.8% | 1289_1290ins132;1312T>C |
| 8 | human | 7525 | YES1 | YES proto-oncogene 1, Src f... | XM_024451247.1 | 91.8% | 91.8% | 1289_1290ins132;1312T>C |
| 9 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_001205132.1 | 91.1% | 95.9% | (many diffs) |
| 10 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_001205133.1 | 91.1% | 95.9% | (many diffs) |
| 11 | mouse | 22612 | Yes1 | YES proto-oncogene 1, Src f... | NM_009535.3 | 91.1% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1698
- ORF length:
- 1629
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgcatt aaaagtaaag aaaacaaaag tccagccatt aaatacagac 121 ctgaaaatac tccagagcct gtcagtacaa gtgtgagcca ttatggagca gaacccacta 181 cagtgtcacc atgtccgtca tcttcagcaa agggaacagc agttaatttc agcagtcttt 241 ccatgacacc atttggagga tcctcagggg taacgccttt tggaggtgca tcttcctcat 301 tttcagtggt gccaagttca tatcctgctg gtttaacagg tggtgttact atatttgtgg 361 ccttatatga ttatgaagct agaactacag aagacctttc atttaagaag ggtgaaagat 421 ttcaaataat taacaatacg gaaggagatt ggtgggaagc aagatcaatc gctacaggaa 481 agaatggtta tatcccgagc aattatgtag cgcctgcaga ttccattcag gcagaagaat 541 ggtattttgg caaaatgggg agaaaagatg ctgaaagatt acttttgaat cctggaaatc 601 aacgaggtat tttcttagta agagagagtg aaacaactaa aggtgcttat tccctttcta 661 ttcgtgattg ggatgagata aggggtgaca atgtgaaaca ctacaaaatt aggaaacttg 721 acaatggtgg atactatatc acaaccagag cacaatttga tactctgcag aaattggtga 781 aacactacac agaacatgct gatggtttat gccacaagtt gacaactgtg tgtccaactg 841 tgaaacctca gactcaaggt ctagcaaaag atgcttggga aatccctcga gaatctttgc 901 gactagaggt taaactagga caaggatgtt tcggcgaagt gtggatggga acatggaatg 961 gaaccacgaa agtagcaatc aaaacactaa aaccaggtac aatgatgcca gaagctttcc 1021 ttcaagaagc tcagataatg aaaaaattaa gacatgataa acttgttcca ctatatgctg 1081 ttgtttctga agaaccaatt tacattgtca ctgaatttat gtcaaaagga agcttattag 1141 atttccttaa ggaaggagat ggaaagtatt tgaagcttcc acagctggtt gatatggctg 1201 ctcagattgc tgatggtatg gcatatattg aaagaatgaa ctatattcac cgagatcttc 1261 gggctgctaa tattcttgta ggagaaaatc ttgtgtgcaa aATAGCAGAC TTTGGTTTAG 1321 CAAGGTTAAT TGAAGACAAT GAATACACAG CAAGACAAGG TGCAAAATTT CCAATCAAAT 1381 GGACAGCTCC TGAAGCTGCA CTGTATGGTC GGTTTACAAT AAAGTCTGAT GTCTGGTCAT 1441 TTGGAATTCT GCAAACAGAA CTAGTAACAA AGGGCCGAGT GCCATATCCA GGTATGGTGA 1501 ACCGTGAAGT ACTAGAACAA GTGGAGCGAG GATACAGGAT GCCGTGCCCT CAGGGCTGTC 1561 CAGAATCCCT CCATGAATTG ATGAATCTGT GTTGGAAGAA GGACCCTGAT GAAAGACCAA 1621 CATTTGAATA TATTCAGTCC TTCTTGGAAG ACTACTTCAC TGCTACAGAG CCACAGTACC 1681 AGCCAGGAGA AAATTTACCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1741 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1801 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACTGTGATC AAACCCTAGA 1861 CCTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt