Construct: ORF TRCN0000471118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018860.2_s317c1
- Derived from:
- ccsbBroadEn_14795
- DNA Barcode:
- GATCACATAAGTACAGACCTGGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCQ (5588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323265.1 | 99.8% | 31.5% | (many diffs) |
| 2 | human | 5588 | PRKCQ | protein kinase C theta | NM_006257.5 | 99.8% | 31.5% | (many diffs) |
| 3 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448076.1 | 99.8% | 31.5% | (many diffs) |
| 4 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252496.4 | 95.2% | 30.1% | (many diffs) |
| 5 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282644.2 | 94.7% | 25.9% | (many diffs) |
| 6 | human | 5588 | PRKCQ | protein kinase C theta | NM_001242413.2 | 90.8% | 34.6% | (many diffs) |
| 7 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252497.4 | 89% | 31.9% | (many diffs) |
| 8 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323267.1 | 88.2% | 27.6% | (many diffs) |
| 9 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282645.1 | 82.1% | 13.7% | (many diffs) |
| 10 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323266.2 | 82.1% | 13.7% | (many diffs) |
| 11 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448077.1 | 82.1% | 13.7% | (many diffs) |
| 12 | mouse | 18761 | Prkcq | protein kinase C, theta | NM_008859.2 | 87% | 30.8% | (many diffs) |
| 13 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497398.3 | 81.9% | 25% | (many diffs) |
| 14 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_011238965.2 | 72.6% | 13.4% | (many diffs) |
| 15 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497399.2 | 55.9% | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 753
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgccattt cttcggattg gcttgtccaa ctttgactgc gggtcctgcc 121 agtcttgtca gggcgaggct gttaaccctt actgtgctgt gctcgtcaaa gagtatgtcg 181 aatcagagaa cgggcagatg tatatccaga aaaagcctac catgtaccca ccctgggaca 241 gcacttttga tgcccatatc aacaagggaa gagtcatgca gatcattgtg aaaggcaaaa 301 acgtggacct catctctgaa accaccgtgg agctctactc gctggctgag aggtgcagga 361 agaacaacgg gaagacagaa atatggttag agctgaaacc tcaaggccga atgctaatga 421 atgcaagata ctttctggaa atgagtgaca caaaggacat gaatgaattt gagacggaag 481 gcttctttgc tttgcatcag cgccggggtg ccatcaagca ggcaaaggtc caccacgtca 541 agtgccacga gttcactgcc accttcttcc cacagcccac attttgctct gtctgccacg 601 agtttgtctg gggcctgaac aaacagggct accagtgccg acaatgcaat gcagcaattc 661 acaagaagtg tattgataaa gttatagcaa agtgcacagg atcagctatc aatagccgag 721 aaaccatgtt ccacaaagga gagattcaaa attgacatgc cacacagatt taaagtctac 781 aattacaaga gcccgacctt ctgtgaacac tgtgggaccc tgctgtgggg actggcacgg 841 caaggactca agtgtgatgc atgtggcatg aatgtgcatc atagatgcca gacaaaggtg 901 gccaaccttt gtggcataaa ccagaagcta atggctgaag cgctggccat gattgagagc 961 actcaacagg ctcgctgctt aagagatact gaacagatct tcagagaagg tccggttgaa 1021 attggtctcc catgctccat caaaaatgaa gcaaggccgc catgtttacc gacaccggga 1081 aaaagagagc ctcagggcat ttcctgggag tctccgttgg atgaggtgga taaaatgtgc 1141 catcttccag aacctgaact gaacaaagaa agaccatctc tgcagattaa actaaaaatt 1201 gaggatttta tcttgcacaa aatgttgggg aaaggaagtt ttggcaaggt cttcctggca 1261 gaattcaaga aaaccaatca atttttcgca ataaaggcct taaagaaaga tgtggtcttg 1321 atggacgatg atgttgagtg cacgatggta gagaagagag ttctttcctt ggcctgggag 1381 catccgtttc tgacgcacat gttttgtaca ttccagacca aggaaaacct ctttttttgt 1441 gatggagtac ctcaacggag gggacttaat gtaccacatc caaagctgcc acaagttcga 1501 cctttccaga gcgacgtttt atgctgctga aatcattctt ggtctgcagt tccttcattc 1561 caaaggaata gtctacaggg acctgaagct agataacatc ctgttagaca aagatggaca 1621 tatcaagatt gcggattttg gaatgtgcaa ggagaacatg ttaggagatg ccaagacgaa 1681 taccttctgt gggacacctg actacatcgc cccagagatc ttgctgggtc agaaatacaa 1741 ccactctgtg gactggtggt ccttcggggt tctcctttat gaaatgctga ttggtcagtc 1801 gcctttccac gggcaggatg aggaggagct cttccactcc atccgcatgg acaatccctt 1861 ttaccCACGG TGGCTGGAGA AGGAAGCAAA GGACCTTCTG GTGAAGCTCT TCGTGAGAGA 1921 ACCTGAGAAG AGGCTGGGCG TGAGGGGAGA CATCCGCCAG CACCCTTTGT TTCGGGAGAT 1981 CAACTGGGAG GAACTTGAAC GGAAGGAGAT TGACCCACCG TTCCGGCCGA AAGTGAAATC 2041 ACCATTTGAC TGCAGCAATT TCGACAAAGA ATTCTTAAAC GAGAAGCCCC GGCTGTCATT 2101 TGCCGACAGA GCACTGATCA ACAGCATGGA CCAGAATATG TTCAGGAACT TTTCCTTCAT 2161 GAACCCCGGG ATGGAGCGGC TGATATCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 2221 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 2281 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATCAC 2341 ATAAGTACAG ACCTGGCCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 2401 gaaagatt