Construct: ORF TRCN0000471123
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010636.1_s317c1
- Derived from:
- ccsbBroadEn_08718
- DNA Barcode:
- AGATCCATGTCGATGCTCTGGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VANGL2 (57216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471123
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57216 | VANGL2 | VANGL planar cell polarity ... | NM_020335.3 | 99.9% | 100% | 1137A>G |
| 2 | human | 57216 | VANGL2 | VANGL planar cell polarity ... | XM_005245357.1 | 99.9% | 100% | 1137A>G |
| 3 | human | 57216 | VANGL2 | VANGL planar cell polarity ... | XM_011509804.1 | 99.9% | 100% | 1137A>G |
| 4 | human | 57216 | VANGL2 | VANGL planar cell polarity ... | XM_011509805.3 | 51.7% | 52.2% | (many diffs) |
| 5 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | NM_033509.4 | 89.9% | 99.4% | (many diffs) |
| 6 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_006497051.3 | 89.9% | 99.4% | (many diffs) |
| 7 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_006497052.3 | 89.9% | 99.4% | (many diffs) |
| 8 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_006497053.3 | 89.9% | 99.4% | (many diffs) |
| 9 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_006497054.3 | 89.9% | 99.4% | (many diffs) |
| 10 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_017313008.1 | 89.9% | 99.4% | (many diffs) |
| 11 | mouse | 93840 | Vangl2 | vang-like 2 (van gogh, Dros... | XM_006497055.2 | 45.2% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1632
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaccgag tcccagtact cgggctattc ctacaagtcg ggccactccc 121 gcagctcccg caagcacagg gaccgccggg accgacaccg ctctaagagt cgagatgggg 181 gccgagggga caagtcggtg acaatccagg ctcccgggga gcccctgctg gacaatgagt 241 ccacacgagg ggatgagcgg gatgacaact ggggggaaac gacgacagta gtaacgggca 301 cctcagagca cagcatctcc catgatgacc tcacacgcat cgccaaggac atggaggaca 361 gtgtccctct ggactgctcc cgtcacctgg gtgtggcagc gggggccacc ctggcactgc 421 tgtctttcct cacgcctctg gccttcctgc tgctgccccc actgctgtgg cgggaggagc 481 tggagccttg cgggacggcc tgcgagggcc tcttcatctc tgtcgccttc aagctgctca 541 tcctgctact gggcagctgg gctctgttct tccgccggcc caaggcctcg ctgccccgcg 601 tctttgtgct gcgtgccctg cttatggtgc tggttttcct gctcgtggtc tcctactggc 661 tcttctatgg tgtgcgcatc ctggatgctc gggagcgcag ctaccagggc gtggtgcagt 721 tcgccgtgtc gctggtggac gcccttcttt tcgtgcacta cctggccgtg gtcctgctgg 781 agctgcgcca gctccagcct cagttcacgc tcaaggtcgt gcgctccacc gacggcgcca 841 gccgcttcta caacgttggc catctcagca tccagcgcgt ggcagtgtgg atcctggaga 901 agtattacca tgacttccct gtctacaacc ctgccctcct caacctgccc aagtccgtcc 961 tggccaagaa agtgtctggc ttcaaggtgt attccctcgg agaggaaaac agcaccaaca 1021 actccactgg ccagtctcgg gctgtgattg cagcggcagc tcggaggcgg gacaacagtc 1081 acaatgagta ctactatgag gaggctgagc atgagcgaag ggtgcgcaag aggagggcca 1141 ggcttgtagt ggcggtggag gaggccttca ctcacattaa gcggctgcag gaagaggagc 1201 agaagaaccc cagggaggtg atggaccccc gggaggcagc ccaagccatc tttgcatcca 1261 tggcccgtgc catgcagaag taccTTCGGA CCACCAAGCA GCAGCCCTAC CACACCATGG 1321 AGAGCATCCT GCAGCACCTT GAATTCTGCA TCACGCATGA CATGACGCCC AAGGCCTTCT 1381 TGGAGCGATA CTTGGCGGCT GGACCTACCA TCCAGTACCA CAAGGAACGC TGGCTGGCCA 1441 AACAGTGGAC ATTGGTGAGC GAGGAGCCGG TGACCAACGG CCTCAAGGAT GGCATCGTTT 1501 TCCTCTTAAA ACGCCAGGAC TTCAGCCTGG TGGTCAGCAC CAAGAAGGTC CCATTCTTCA 1561 AACTCTCCGA GGAATTTGTG GATCCCAAGT CACACAAGTT TGTCATGAGG CTGCAGTCTG 1621 AGACCTCAGT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGA TCCATGTCGA TGCTCTGGCT 1801 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t