Construct: ORF TRCN0000471130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016433.1_s317c1
- Derived from:
- ccsbBroadEn_01439
- DNA Barcode:
- CAGTTAACCCTCCGACACTTCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL37A (6168)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471130
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6168 | RPL37A | ribosomal protein L37a | NM_000998.5 | 100% | 100% | |
| 2 | mouse | 19981 | Rpl37a | ribosomal protein L37a | NM_009084.4 | 90.5% | 100% | (many diffs) |
| 3 | mouse | 70873 | Cnbd2 | cyclic nucleotide binding d... | XM_017319256.1 | 29.7% | 31.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 366
- ORF length:
- 276
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCAAACG TACCAAGAAA GTCGGGATCG 121 TCGGTAAATA CGGGACCCGC TATGGGGCCT CCCTCCGGAA AATGGTGAAG AAAATTGAAA 181 TCAGCCAGCA CGCCAAGTAC ACTTGCTCTT TCTGTGGCAA AACCAAGATG AAGAGACGAG 241 CTGTGGGGAT CTGGCACTGT GGTTCCTGCA TGAAGACAGT GGCTGGCGGT GCCTGGACGT 301 ACAATACCAC TTCCGCTGTC ACGGTAAAGT CCGCCATCAG AAGACTGAAG GAGTTGAAAG 361 ACCAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGTTAACC CTCCGACACT TCCCTACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt