Construct: ORF TRCN0000471154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012376.1_s317c1
- Derived from:
- ccsbBroadEn_00613
- DNA Barcode:
- AATTATGGTTGCATTTCTCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT3 (2591)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471154
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2591 | GALNT3 | polypeptide N-acetylgalacto... | NM_004482.4 | 100% | 100% | |
| 2 | human | 2591 | GALNT3 | polypeptide N-acetylgalacto... | XM_005246449.1 | 100% | 100% | |
| 3 | human | 2591 | GALNT3 | polypeptide N-acetylgalacto... | XM_011510929.1 | 100% | 100% | |
| 4 | human | 2591 | GALNT3 | polypeptide N-acetylgalacto... | XM_017003770.1 | 100% | 100% | |
| 5 | human | 2591 | GALNT3 | polypeptide N-acetylgalacto... | XR_002959253.1 | 71.1% | 1_341del;1532_1533ins65;1935_1936ins240 | |
| 6 | mouse | 14425 | Galnt3 | UDP-N-acetyl-alpha-D-galact... | NM_015736.2 | 87% | 91.9% | (many diffs) |
| 7 | mouse | 14425 | Galnt3 | UDP-N-acetyl-alpha-D-galact... | XR_374409.3 | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1968
- ORF length:
- 1899
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcaccta aagcgactag taaaattaca cattaaaaga cattaccata 121 aaaagttctg gaagcttggt gcagtaattt ttttctttat aatagttttg gttttaatgc 181 aaagagaagt aagtgttcaa tattccaaag aggaatcaag gatggaaagg aacatgaaaa 241 acaaaaacaa gatgttggat ttaatgctag aagctgtaaa caatattaag gatgccatgc 301 caaaaatgca aataggagca cctgtcaggc aaaacattga tgctggtgag agaccttgtt 361 tgcaaggata ttatacagca gcagaattga agcctgtcct tgaccgtcca cctcaggatt 421 caaatgcacc tggtgcttct ggtaaagcat tcaagacaac caatttaagt gttgaagagc 481 aaaaggaaaa ggaacgtggg gaagctaaac actgctttaa tgctttcgca agtgacagga 541 tttctttgca ccgagatctt ggaccagaca ctcgacctcc tgaatgtatt gaacaaaaat 601 ttaagcgctg ccctcccctg cccaccacca gtgtcataat agtttttcat aatgaagcgt 661 ggtccacgtt gcttagaact gtccacagtg tgctctattc ttcacctgca atactgctga 721 aggaaatcat tttggtggat gatgctagtg tagatgagta cttacatgat aaactagatg 781 aatatgtaaa acaattttct atagtaaaaa tagtcagaca aagagaaaga aaaggtctga 841 tcactgctcg gttgctagga gcaacagtcg caacagctga aacgctcaca tttttagatg 901 ctcactgtga gtgtttctat ggttggctag aacctctgtt ggccagaata gctgagaact 961 acacggctgt cgtaagtcca gatattgcat ccatagatct gaacacgttt gaattcaaca 1021 aaccttctcc ttatggaagt aaccataacc gtggaaattt tgactggagt ctttcatttg 1081 gctgggagtc gcttcctgat catgagaagc aaagaaggaa agatgaaacc tacccaatta 1141 aaacacccac ttttgcagga ggactttttt ccatatcaaa agaatatttt gagtatattg 1201 gaagctatga tgaagaaatg gaaatctggg gaggtgaaaa tatagaaatg tctttcagag 1261 tatggcaatg tggtgggcag ttggagatta tgccttgctc tgttgttgga catgtttttc 1321 gcagcaaaag ccctcatagc tttccaaaag gcactcaggt gattgctaga aaccaagttc 1381 gccttgcaga agtctggatg gatgaataca aggaaatatt ttataggaga aatacagatg 1441 cagcaaaaat tgttaaacaa aaagcatttg gtgatctttc aaaaagattt gaaataaaac 1501 accgccttca gtGTAAAAAT TTTACATGGT ATCTGAACAA CATTTATCCA GAGGTGTATG 1561 TGCCAGACCT TAATCCTGTT ATATCTGGAT ACATTAAAAG CGTTGGTCAG CCTCTATGTC 1621 TGGATGTTGG AGAAAACAAT CAAGGAGGCA AACCATTAAT TATGTATACA TGTCATGGAC 1681 TTGGGGGAAA CCAGTACTTT GAATACTCTG CTCAACATGA AATTCGGCAC AACATCCAGA 1741 AGGAATTATG TCTTCATGCT GCTCAAGGTC TCGTTCAGCT GAAGGCATGT ACCTACAAAG 1801 GTCACAAGAC AGTTGTCACT GGAGAGCAGA TATGGGAGAT CCAGAAGGAT CAACTTCTAT 1861 ACAATCCATT CTTAAAAATG TGCCTTTCAG CAAATGGAGA GCATCCAAGT TTAGTGTCAT 1921 GCAACCCATC AGATCCACTC CAAAAATGGA TACTTAGCCA AAATGATTTG CCAACTTTCT 1981 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2041 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2101 GTGGAAAGGA CGAAATTATG GTTGCATTTC TCCAATCACG CGTTAAGTCg acaatcaacc 2161 tctggattac aaaatttgtg aaagatt