Construct: ORF TRCN0000471158
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013557.1_s317c1
- Derived from:
- ccsbBroadEn_01403
- DNA Barcode:
- CCTACATTAGCGGATGTTGTTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RLN2 (6019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471158
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6019 | RLN2 | relaxin 2 | NM_134441.3 | 100% | 100% | |
| 2 | human | 6013 | RLN1 | relaxin 1 | XM_024447637.1 | 90.4% | 89.7% | (many diffs) |
| 3 | human | 6019 | RLN2 | relaxin 2 | XM_024447646.1 | 90.4% | 89.7% | (many diffs) |
| 4 | human | 6019 | RLN2 | relaxin 2 | XM_024447647.1 | 90.4% | 89.7% | (many diffs) |
| 5 | human | 6013 | RLN1 | relaxin 1 | NM_006911.4 | 90.4% | 82.1% | (many diffs) |
| 6 | human | 6013 | RLN1 | relaxin 1 | XM_024447638.1 | 85.4% | 85.4% | 0_1ins81 |
| 7 | human | 6013 | RLN1 | relaxin 1 | XM_017015007.1 | 66.3% | 53.1% | (many diffs) |
| 8 | human | 6013 | RLN1 | relaxin 1 | XM_017015008.1 | 61.1% | 53.4% | (many diffs) |
| 9 | human | 6019 | RLN2 | relaxin 2 | NM_001329191.1 | 54% | 54% | 0_1ins255 |
| 10 | human | 6013 | RLN1 | relaxin 1 | XM_024447639.1 | 54% | 54% | 0_1ins255 |
| 11 | human | 6013 | RLN1 | relaxin 1 | XM_024447640.1 | 54% | 54% | 0_1ins255 |
| 12 | human | 6013 | RLN1 | relaxin 1 | XM_024447641.1 | 54% | 54% | 0_1ins255 |
| 13 | human | 6013 | RLN1 | relaxin 1 | XM_017015010.1 | 48.1% | 34.3% | (many diffs) |
| 14 | human | 6013 | RLN1 | relaxin 1 | XM_024447642.1 | 46.5% | 41% | (many diffs) |
| 15 | human | 6013 | RLN1 | relaxin 1 | XM_024447643.1 | 46.5% | 41% | (many diffs) |
| 16 | human | 6013 | RLN1 | relaxin 1 | XM_024447644.1 | 46.5% | 41% | (many diffs) |
| 17 | human | 6013 | RLN1 | relaxin 1 | XM_024447645.1 | 46.5% | 41% | (many diffs) |
| 18 | human | 6019 | RLN2 | relaxin 2 | NM_005059.3 | 38.1% | 41.8% | 212_312del;351_352ins305 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 624
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcgcctg ttttttttcc acctgctagg agtctgttta ctactgaacc 121 aattttccag agcagtcgcg gactcatGGA TGGAGGAAGT TATTAAATTA TGCGGCCGCG 181 AATTAGTTCG CGCGCAGATT GCCATTTGCG GCATGAGCAC CTGGAGCAAA AGGTCTCTGA 241 GCCAGGAAGA TGCTCCTCAG ACACCTAGAC CAGTGGCAGA AATTGTGCCA TCCTTCATCA 301 ACAAAGATAC AGAAACCATA AATATGATGT CAGAATTTGT TGCTAATTTG CCACAGGAGC 361 TGAAGTTAAC CCTGTCTGAG ATGCAGCCAG CATTACCACA GCTACAACAA CATGTACCTG 421 TATTAAAAGA TTCCAGTCTT CTCTTTGAAG AATTTAAGAA ACTTATTCGC AATAGACAAA 481 GTGAAGCCGC AGACAGCAGT CCTTCAGAAT TAAAATACTT AGGCTTGGAT ACTCATTCTC 541 GAAAAAAGAG ACAACTCTAC AGTGCATTGG CTAATAAATG TTGCCATGTT GGTTGTACCA 601 AAAGATCTCT TGCTAGATTT TGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTACATTAGC 781 GGATGTTGTT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att