Transcript: Human XM_024447644.1

PREDICTED: Homo sapiens relaxin 1 (RLN1), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RLN1 (6013)
Length:
724
CDS:
143..442

Additional Resources:

NCBI RefSeq record:
XM_024447644.1
NBCI Gene record:
RLN1 (6013)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024447644.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000152072 CACACATAGTCTTGATGACAT pLKO.1 477 3UTR 100% 4.950 3.465 N RLN1 n/a
2 TRCN0000150330 CCTGCATTAAAGGATTCCAAT pLKO.1 233 CDS 100% 4.950 3.465 N RLN1 n/a
3 TRCN0000154049 CAACCATCATTACCAGAGCTA pLKO.1 200 CDS 100% 2.640 1.848 N RLN1 n/a
4 TRCN0000150626 GCTTGGATACTCATTCTCAAA pLKO.1 339 CDS 100% 4.950 2.970 N RLN1 n/a
5 TRCN0000151339 GATGACATTTCACTGATGCTT pLKO.1 490 3UTR 100% 3.000 1.800 N RLN1 n/a
6 TRCN0000151006 GTACCATCCTTCATCAACAAA pLKO.1 101 5UTR 100% 0.000 0.000 N RLN1 n/a
7 TRCN0000120356 CCATCCTTCATCAACAAAGAT pLKO.1 104 5UTR 100% 5.625 2.813 Y Rln1 n/a
8 TRCN0000146706 CCATCCTTCATCAACAAAGAT pLKO.1 104 5UTR 100% 5.625 2.813 Y RLN2 n/a
9 TRCN0000150355 CCATCCTTCATCAACAAAGAT pLKO.1 104 5UTR 100% 5.625 2.813 Y RLN1 n/a
10 TRCN0000152386 GAAGCTAATTGTGCACATCTT pLKO.1 446 3UTR 100% 4.950 2.475 Y RLN1 n/a
11 TRCN0000151299 GATGAAGCTAATTGTGCACAT pLKO.1 443 CDS 100% 4.050 2.025 Y RLN1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024447644.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01399 pDONR223 100% 53.5% 53.5% None 0_1ins258 n/a
2 TRCN0000466101 TAATCCGTTCGAACCTTCCAGGAA pLX_317 59.8% 53.5% 53.5% V5 0_1ins258 n/a
3 ccsbBroadEn_01403 pDONR223 100% 46.5% 41% None (many diffs) n/a
4 ccsbBroad304_01403 pLX_304 0% 46.5% 41% V5 (many diffs) n/a
5 TRCN0000471158 CCTACATTAGCGGATGTTGTTCTG pLX_317 84.2% 46.5% 41% V5 (many diffs) n/a
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