Construct: ORF TRCN0000471162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017670.1_s317c1
- Derived from:
- ccsbBroadEn_08793
- DNA Barcode:
- TATGGGGGAAAAATCAGCCTTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOC4 (60412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60412 | EXOC4 | exocyst complex component 4 | NM_001037126.1 | 99.7% | 99.7% | 1419_1419delGinsTGAG |
| 2 | human | 60412 | EXOC4 | exocyst complex component 4 | XM_005250523.5 | 97.6% | 97.3% | 1418_1421delCATCinsG;1424_1429delCTGCCA;1432_1455del |
| 3 | human | 60412 | EXOC4 | exocyst complex component 4 | NM_021807.4 | 48.6% | 48.5% | 1419_1424delGGGTCC;1428_2922delinsG |
| 4 | human | 60412 | EXOC4 | exocyst complex component 4 | XR_001744845.2 | 35.1% | 1_27del;1446_1451delGGGTCC;1455_4042delinsG | |
| 5 | mouse | 20336 | Exoc4 | exocyst complex component 4 | NM_001347089.1 | 79.2% | 85.2% | (many diffs) |
| 6 | mouse | 20336 | Exoc4 | exocyst complex component 4 | XM_011241262.1 | 55.9% | 60.2% | (many diffs) |
| 7 | mouse | 20336 | Exoc4 | exocyst complex component 4 | NM_009148.3 | 42.4% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1488
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagaagca gctggtggga aatacagaag cacagtcagc aaaagcaaag 121 acccctcggg gctgctcatc tctgtgatca ggactctgtc tactagtgac gatgtcgaag 181 acagggaaaa tgaaaagggt cgccttgaag aagcctacga gaaatgtgac cgtgacctgg 241 atgaattgat tgtacagcac tacacagaat tgacgacagc cattcgcaca taccagagca 301 tcacagagcg catcactaac tcccgaaata aaataaagca ggtaaaagag aacctgcttt 361 catgcaagat gctgctgcac tgcaaacggg atgagcttcg gaaactgtgg attgaaggaa 421 ttgagcataa gcatgtcctg aacttgttgg atgaaattga gaatatcaag caagtgcctc 481 aaaagctgga acagtgcatg gccagcaagc actatctcag tgccactgac atgttggtgt 541 cagcagttga gtctttggag ggccccctgc tccaggtgga aggactgagt gaccttcgac 601 tagagcttca cagcaagaag atgaaccttc acttggttct catagatgaa ctacaccggc 661 acctgtacat caaatcgact agccgagttg tgcagcgtaa caaggaaaaa gggaaaatca 721 gctccctcgt gaaagatgct tctgttcctc tgattgatgt tacaaacctc cctactcctc 781 gaaaattcct tgatacctct cactattcta ctgctggaag ctcaagtgtg agggagataa 841 atctgcagga catcaaggaa gatttagaat tggatccaga ggaaaacagc accctgttta 901 tgggtatcct cattaagggc ttggcgaaac tgaagaagat cccagaaaca gttaaggcaa 961 tcatagagcg cttggagcag gagttGAAGC AAATTGTGAA GAGGTCTACA ACCCAGGTGG 1021 CAGACAGTGG CTATCAGCGG GGGGAGAACG TTACTGTGGA GAACCAACCA AGGTTGCTTC 1081 TAGAACTGCT GGAGTTACTG TTTGACAAGT TTAATGCTGT AGCCGCTGCA CACTCTGTGG 1141 TCCTGGGATA CCTGCAGGAC ACTGTAGTGA CTCCACTGAC TCAGCAGGAA GATATCAAAC 1201 TGTATGATAT GGCAGATGTA TGGGTGAAGA TCCAAGATGT TCTACAGATG CTATTAACTG 1261 AGTACTTGGA TATGAAAAAT ACTCGTACGG CCTCTGAACC ATCAGCTCAA CTAAGCTATG 1321 CCAGCACTGG ACGAGAGTTT GCAGCCTTTT TTGCCAAGAA GAAACCTCAA AGGCCAAAAA 1381 ATTCTCTTTT CAAGTTCGAA TCGTCCTCCC ATGCCATCAG TATGAGCGCC TATCTGCGAG 1441 AACAGAGAAG GGAGCTCTAT AGTCGGAGTG GAGAACTGCA AGGTGAGTGC CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGATATGGGG GAAAAATCAG CCTTACTACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt