Construct: ORF TRCN0000471185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004912.1_s317c1
- Derived from:
- ccsbBroadEn_08787
- DNA Barcode:
- CATTTGACGTCAGCAGCGCAACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL12 (59349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59349 | KLHL12 | kelch like family member 12 | NM_021633.4 | 100% | 100% | |
| 2 | human | 59349 | KLHL12 | kelch like family member 12 | NM_001303051.2 | 93.7% | 93.7% | 1_114del |
| 3 | human | 59349 | KLHL12 | kelch like family member 12 | XM_011509835.2 | 91.1% | 91.1% | 1_165del |
| 4 | human | 59349 | KLHL12 | kelch like family member 12 | XM_017001995.2 | 82.3% | 81.8% | (many diffs) |
| 5 | human | 59349 | KLHL12 | kelch like family member 12 | NM_001303109.2 | 82.2% | 82.2% | 830_831ins303 |
| 6 | human | 59349 | KLHL12 | kelch like family member 12 | XM_011509836.2 | 75% | 74.6% | (many diffs) |
| 7 | human | 59349 | KLHL12 | kelch like family member 12 | XM_011509837.2 | 74.9% | 74.9% | 1_165del;995_996ins303 |
| 8 | mouse | 240756 | Klhl12 | kelch-like 12 | NM_001311136.1 | 91.3% | 99.2% | (many diffs) |
| 9 | mouse | 240756 | Klhl12 | kelch-like 12 | XM_006529561.2 | 91.3% | 99.2% | (many diffs) |
| 10 | mouse | 240756 | Klhl12 | kelch-like 12 | NM_153128.3 | 86.7% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1770
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aggcattatg gcccccaaag acataatgac aaatactcat gctaaatcca 121 tcctcaattc aatgaactcc ctcaggaaga gcaataccct ctgtgatgtg acattgagag 181 tagagcagaa agacttccct gcccatcgga ttgtgctggc tgcctgtagt gattacttct 241 gtgccatgtt cactagtgag ctctcagaga aggggaaacc ttatgttgac atccaaggtt 301 tgactgcctc taccatggaa attttattgg actttgtgta cacagaaaca gtacatgtga 361 cagtggagaa tgtacaagaa ctgcttcctg cagcctgtct gcttcagttg aaaggtgtga 421 aacaagcctg ctgtgagttc ttagaaagtc agttggaccc ttctaattgc ctgggtatta 481 gggattttgc tgaaacccac aattgtgttg acctgatgca agcagctgag gtttttagcc 541 agaagcattt tcctgaagtg gtacagcatg aagagttcat tcttctgagt caaggagagg 601 tggaaaagct aatcaagtgc gacgaaattc aggtggattc tgaagagcca gtctttgagg 661 ctgtcatcaa ctgggtgaag catgccaaga aagagcggga agaatccttg cctaacctgc 721 tacagtatgt gcggatgccc ctactaaccc ccaggtatat cacagatgta atagatgctg 781 agcctttcat ccgctgtagt ttacaatgca gggatctggt tgatgaagca aagaagtttc 841 atctgaggcc tgaacttcgg agtcagatgc agggacccag gacaagggct cgcctaggag 901 ccaatgaagt gcttttggtg gttgggggct ttggaagcca gcagtctccc attgatgtgg 961 tagagaaata tgaccccaag actcaggagt ggagcttttt gccaagcatc actcgtaaga 1021 gacgttatgt ggcctcagtg tcccttcatg accggatcta cgtcattggt ggctatgatg 1081 gccgttcccg ccttagttca gtggaatgtc tagactacac agcagatgag gatggggtct 1141 ggtattctgt ggcccctatg aatgtccgac gaggtcttgc tggagccacc accctgggag 1201 atatgatcta tgtctctgga ggctttgatg gaagcaggcg tcacaccagt atggagcgct 1261 atgatccaaa cattgaccag tggagcatgc tgggagatat gcagacagcc cgggaaggtg 1321 ccggactcgt agtggccagt ggagtgatct actgtctagg aggatatgac ggcttgaata 1381 TCTTAAATTC AGTTGAGAAA TACGACCCTC ATACAGGACA TTGGACTAAT GTTACACCAA 1441 TGGCCACCAA GCGTTCTGGT GCAGGAGTAG CCCTGCTGAA TGACCATATT TATGTGGTGG 1501 GGGGATTTGA TGGTACAGCC CACCTTTCTT CCGTTGAAGC ATACAACATT CGCACTGATT 1561 CCTGGACAAC TGTCACCAGT ATGACCACTC CACGATGCTA TGTAGGGGCC ACAGTGCTTC 1621 GGGGGAGACT CTATGCAATT GCAGGATATG ATGGTAATTC CCTGCTAAGT AGCATTGAAT 1681 GTTATGACCC TATCATCGAC AGCTGGGAAG TCGTGACATC CATGGGAACC CAGCGCTGTG 1741 ATGCTGGTGT TTGTGTTCTC CGCGAGAAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACATTT 1921 GACGTCAGCA GCGCAACTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt