Transcript: Human XM_011509835.2

PREDICTED: Homo sapiens kelch like family member 12 (KLHL12), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLHL12 (59349)
Length:
3368
CDS:
80..1951

Additional Resources:

NCBI RefSeq record:
XM_011509835.2
NBCI Gene record:
KLHL12 (59349)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011509835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276598 ACTATGTGCACCGGGTGATTA pLKO_005 2042 3UTR 100% 13.200 18.480 N KLHL12 n/a
2 TRCN0000276547 TGACGGCTTGAATATCTTAAA pLKO_005 1546 CDS 100% 13.200 18.480 N KLHL12 n/a
3 TRCN0000154284 GCCTACTAGATATGGGCAATA pLKO.1 2310 3UTR 100% 10.800 15.120 N KLHL12 n/a
4 TRCN0000154237 CATCACTCGTAAGAGACGTTA pLKO.1 1186 CDS 100% 4.950 6.930 N KLHL12 n/a
5 TRCN0000152391 GCAAAGAACTAAGGTGAGTAT pLKO.1 2288 3UTR 100% 4.950 6.930 N KLHL12 n/a
6 TRCN0000157060 GCGACGAAATTCAGGTGGATT pLKO.1 798 CDS 100% 4.950 6.930 N KLHL12 n/a
7 TRCN0000276546 GCGACGAAATTCAGGTGGATT pLKO_005 798 CDS 100% 4.950 6.930 N KLHL12 n/a
8 TRCN0000276597 CATACAGGACATTGGACTAAT pLKO_005 1589 CDS 100% 13.200 10.560 N KLHL12 n/a
9 TRCN0000152341 CCTGTCCTTTAGTGTAGATTT pLKO.1 3069 3UTR 100% 13.200 9.240 N KLHL12 n/a
10 TRCN0000152342 CTTCTAATTGCCTGGGTATTA pLKO.1 639 CDS 100% 13.200 9.240 N KLHL12 n/a
11 TRCN0000152220 CCAGGTATATCACAGATGTAA pLKO.1 930 CDS 100% 5.625 3.938 N KLHL12 n/a
12 TRCN0000154087 CCCTGCTAAGTAGCATTGAAT pLKO.1 1839 CDS 100% 5.625 3.938 N KLHL12 n/a
13 TRCN0000108923 GCCTGCTGTGAGTTCTTAGAA pLKO.1 605 CDS 100% 5.625 3.938 N Klhl12 n/a
14 TRCN0000153747 CCCAGGTATATCACAGATGTA pLKO.1 929 CDS 100% 4.950 3.465 N KLHL12 n/a
15 TRCN0000158347 CTGGACAACTGTCACCAGTAT pLKO.1 1741 CDS 100% 4.950 3.465 N KLHL12 n/a
16 TRCN0000153283 GCATACAACATTCGCACTGAT pLKO.1 1718 CDS 100% 4.950 3.465 N KLHL12 n/a
17 TRCN0000276599 CCCTGCTGAATGACCATATTT pLKO_005 1650 CDS 100% 15.000 9.000 N KLHL12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011509835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08787 pDONR223 100% 91.1% 91.1% None 1_165del n/a
2 ccsbBroad304_08787 pLX_304 0% 91.1% 91.1% V5 1_165del n/a
3 TRCN0000471185 CATTTGACGTCAGCAGCGCAACTT pLX_317 23.2% 91.1% 91.1% V5 1_165del n/a
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