Construct: ORF TRCN0000471195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002951.1_s317c1
- Derived from:
- ccsbBroadEn_13305
- DNA Barcode:
- ACTAATGTCAGATACCACCTCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF483 (158399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471195
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158399 | ZNF483 | zinc finger protein 483 | XM_017014339.1 | 100% | 100% | |
| 2 | human | 158399 | ZNF483 | zinc finger protein 483 | NM_001007169.5 | 94.7% | 94.5% | (many diffs) |
| 3 | human | 158399 | ZNF483 | zinc finger protein 483 | NM_133464.5 | 32.6% | 32.2% | (many diffs) |
| 4 | human | 158399 | ZNF483 | zinc finger protein 483 | XM_011518300.2 | 32.6% | 32.2% | (many diffs) |
| 5 | human | 158399 | ZNF483 | zinc finger protein 483 | XM_017014337.1 | 32.6% | 32.2% | (many diffs) |
| 6 | human | 158399 | ZNF483 | zinc finger protein 483 | XM_017014338.1 | 32.6% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca agctgtagtg cccttgaaca agatgacagc catctcacca gaacctcaaa 121 ctctggcctc gactgaacaa aatgaggtcc caagagtggt tacttctggg gaacaagaag 181 ctattttaag aggaaatgct gctgatgcag agtctttcag acagaggttt aggtggtttt 241 gttactcaga agtagctgga cccaggaaag ctctgagtca actctgggag ctctgcaatc 301 agtggctgag accagacatt cacacgaaag aacagatttt agagcttctg gtgtttgagc 361 agttcctgac cattttgcct ggggagatca ggatttgggt aaagtcacaa catcctgaga 421 gtagtgagga agtggtgacc ctaatagaag atttgaccca gatgcttgaa gaaaaagatc 481 cagtctctca agattctact gtttcccaag aggagaactc aaaagaggat aaaatggtca 541 ctgtttgtcc caatacTGAG TCCTGTGAAT CTATAACATT GAAGGATGTA GCTGTGAACT 601 TTTCAAGAGG AGAGTGGAAG AAGCTGGAGC CTTTTCAAAA GGAGCTATAT AAGGAAGTGC 661 TACTGGAAAA CCTCAGGAAC CTAGAATTTC TGGACTTTCC AGTTTCAAAA TTAGAGTTGA 721 TTTCCCAGCT AAAGTGGGTT GAATTGCCAT GGCTGCTGGA AGAAGTCTCA AAAAGCTCCC 781 GACTAGGAAG TGTGATTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTAATG TCAGATACCA 961 CCTCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt