Construct: ORF TRCN0000471203

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017640.1_s317c1
Derived from:
ccsbBroadEn_01311
DNA Barcode:
CGCCACATCTCCACATATGGGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMB3 (5691)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471203
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5691 PSMB3 proteasome 20S subunit beta 3 NM_002795.4 100% 100%
2 human 5691 PSMB3 proteasome 20S subunit beta 3 NR_104195.2 70.6% 1_86del;381_382ins77;625_685del
3 human 5691 PSMB3 proteasome 20S subunit beta 3 NR_104194.2 57.3% 1_86del;381_382ins178;524_584del
4 mouse 26446 Psmb3 proteasome (prosome, macrop... NM_011971.4 91% 98.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
681
ORF length:
615
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttcgcatgtc tattatgtcc tataacggag gggccgtcat ggccatgaag gggaagaact
121gtgtggccat cgctgcagac aggcgcttcg ggatccaggc ccagatggtg accacggact
181tccagaagat ctttcccatg ggtgaccggc tgtacatcgg tctggccggg ctcgccactg
241acgtccagac agttgcccag cgcctcaagt tccggctgaa cctgtatgag ttGAAGGAAG
301GTCGGCAGAT CAAACCTTAT ACCCTCATGA GCATGGTGGC CAACCTCTTG TATGAGAAAC
361GGTTTGGCCC TTACTACACT GAGCCAGTCA TTGCCGGGTT GGACCCGAAG ACCTTTAAGC
421CCTTCATTTG CTCTCTAGAC CTCATCGGCT GCCCCATGGT GACTGATGAC TTTGTGGTCA
481GTGGCACCTG CGCCGAACAA ATGTACGGAA TGTGTGAGTC CCTCTGGGAG CCCAACATGG
541ATCCGGATCA CCTGTTTGAA ACCATCTCCC AAGCCATGCT GAATGCTGTG GACCGGGATG
601CAGTGTCAGG CATGGGAGTC ATTGTCCACA TCATCGAGAA GGACAAAATC ACCACCAGGA
661CACTGAAGGC CCGAATGGAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
721CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
781AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGCC ACATCTCCAC
841ATATGGGTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)