Construct: ORF TRCN0000471203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017640.1_s317c1
- Derived from:
- ccsbBroadEn_01311
- DNA Barcode:
- CGCCACATCTCCACATATGGGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMB3 (5691)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471203
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5691 | PSMB3 | proteasome 20S subunit beta 3 | NM_002795.4 | 100% | 100% | |
| 2 | human | 5691 | PSMB3 | proteasome 20S subunit beta 3 | NR_104195.2 | 70.6% | 1_86del;381_382ins77;625_685del | |
| 3 | human | 5691 | PSMB3 | proteasome 20S subunit beta 3 | NR_104194.2 | 57.3% | 1_86del;381_382ins178;524_584del | |
| 4 | mouse | 26446 | Psmb3 | proteasome (prosome, macrop... | NM_011971.4 | 91% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttcgcatgtc tattatgtcc tataacggag gggccgtcat ggccatgaag gggaagaact 121 gtgtggccat cgctgcagac aggcgcttcg ggatccaggc ccagatggtg accacggact 181 tccagaagat ctttcccatg ggtgaccggc tgtacatcgg tctggccggg ctcgccactg 241 acgtccagac agttgcccag cgcctcaagt tccggctgaa cctgtatgag ttGAAGGAAG 301 GTCGGCAGAT CAAACCTTAT ACCCTCATGA GCATGGTGGC CAACCTCTTG TATGAGAAAC 361 GGTTTGGCCC TTACTACACT GAGCCAGTCA TTGCCGGGTT GGACCCGAAG ACCTTTAAGC 421 CCTTCATTTG CTCTCTAGAC CTCATCGGCT GCCCCATGGT GACTGATGAC TTTGTGGTCA 481 GTGGCACCTG CGCCGAACAA ATGTACGGAA TGTGTGAGTC CCTCTGGGAG CCCAACATGG 541 ATCCGGATCA CCTGTTTGAA ACCATCTCCC AAGCCATGCT GAATGCTGTG GACCGGGATG 601 CAGTGTCAGG CATGGGAGTC ATTGTCCACA TCATCGAGAA GGACAAAATC ACCACCAGGA 661 CACTGAAGGC CCGAATGGAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGCC ACATCTCCAC 841 ATATGGGTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt