Construct: ORF TRCN0000471204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004755.1_s317c1
- Derived from:
- ccsbBroadEn_01432
- DNA Barcode:
- CGAAAGAAACAAATAACCACCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL28 (6158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6158 | RPL28 | ribosomal protein L28 | NM_000991.5 | 100% | 100% | |
| 2 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001363697.1 | 76.3% | 65.1% | (many diffs) |
| 3 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136134.1 | 75.8% | 65.6% | (many diffs) |
| 4 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136135.2 | 75.5% | 64.2% | (many diffs) |
| 5 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136136.1 | 52.6% | 50.3% | (many diffs) |
| 6 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136137.1 | 50.3% | 49.6% | 205_206insGCC;207_208ins201 |
| 7 | mouse | 19943 | Rpl28 | ribosomal protein L28 | NM_009081.2 | 89.2% | 98.5% | (many diffs) |
| 8 | mouse | 100039731 | Gm10051 | predicted pseudogene 10051 | XR_868574.1 | 74.3% | (many diffs) | |
| 9 | mouse | 19943 | Rpl28 | ribosomal protein L28 | XM_006539679.1 | 74.1% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 501
- ORF length:
- 411
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGCGCA TCTGCAATGG ATGGTCGTGC 121 GGAACTGCTC CAGTTTCCTG ATCAAGAGGA ATAAGCAGAC CTACAGCACT GAGCCCAATA 181 ACTTGAAGGC CCGCAATTCC TTCCGCTACA ACGGACTGAT TCACCGCAAG ACTGTGGGCG 241 TGGAGCCGGC AGCCGACGGC AAAGGTGTCG TGGTGGTCAT TAAGCGGAGA TCCGGCCAGC 301 GGAAGCCTGC CACCTCCTAT GTGCGGACCA CCATCAACAA GAATGCTCGC GCCACGCTCA 361 GCAGCATCAG ACACATGATC CGCAAGAACA AGTACCGCCC CGACCTGCGC ATGGCAGCCA 421 TCCGCAGGGC CAGCGCCATC CTGCGCAGCC AGAAGCCTGT GATGGTGAAG AGGAAGCGGA 481 CCCGCCCCAC CAAGAGCTCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGAA AGAAACAAAT 661 AACCACCTCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt