Construct: ORF TRCN0000471223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007361.1_s317c1
- Derived from:
- ccsbBroadEn_01194
- DNA Barcode:
- ATCCTGAGCATCCAATCCCGTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINE2 (5270)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5270 | SERPINE2 | serpin family E member 2 | NM_001136528.2 | 100% | 100% | |
| 2 | human | 5270 | SERPINE2 | serpin family E member 2 | XM_017004332.2 | 100% | 100% | |
| 3 | human | 5270 | SERPINE2 | serpin family E member 2 | NM_006216.3 | 99.7% | 99.4% | 986_988delCAG |
| 4 | human | 5270 | SERPINE2 | serpin family E member 2 | XM_017004330.1 | 99.7% | 99.4% | 986_988delCAG |
| 5 | human | 5270 | SERPINE2 | serpin family E member 2 | NM_001136530.1 | 97% | 97% | 1_36del |
| 6 | human | 5270 | SERPINE2 | serpin family E member 2 | XM_005246641.2 | 96.8% | 96.5% | 1_36del;1022_1024delCAG |
| 7 | human | 5270 | SERPINE2 | serpin family E member 2 | NR_073116.1 | 44.5% | 1_661del;1853_2674del | |
| 8 | mouse | 20720 | Serpine2 | serine (or cysteine) peptid... | NM_009255.4 | 84.1% | 85.3% | (many diffs) |
| 9 | mouse | 20720 | Serpine2 | serine (or cysteine) peptid... | XM_006496459.3 | 84.1% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1257
- ORF length:
- 1191
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctggcatctc cccctcttcc tcttggcctc tgtgacgctg ccttccatct 121 gctcccactt caatcctctg tctctcgagg aactaggctc caacacgggg atccaggttt 181 tcaatcagat tgtgaagtcg aggcctcatg acaacatcgt gatctctccc catgggattg 241 cgtcggtcct ggggatgctt cagctggggg cggacggcag gaccaagaag cagctcgcca 301 tggtgatgag atacggcgta aatggagttg gtaaaatatt aaagaagatc aacaaggcca 361 tcgtctccaa gaagaataaa gacattgtga cagtggctaa cgccgtgttt gttaagaatg 421 cctctgaaat tgaagtgcct tttgttacaa ggaacaaaga tgtgttccag tgtgaggtcc 481 ggaatgtgaa ctttgaggat ccagcctctg cctgtgattc catcaatgca tgggttaaaa 541 atgaaaccag ggatatgatt gacaatctgc tgtccccaga tcttattgat ggtgtgctca 601 ccagactggt cctcgtcaac gcagtgtatt tcaagggtct gtggaaatca cggttccaac 661 ccgagaacac aaagaaacgc actttcgtgg cagccgacgg gaaatcctat caagtgccaa 721 tgctggccca gctctccgtg ttccggtgtg ggtcGACAAG TGCCCCCAAT GATTTATGGT 781 ACAACTTCAT TGAACTGCCC TACCACGGGG AAAGCATCAG CATGCTGATT GCACTGCCGA 841 CTGAGAGCTC CACTCCGCTG TCTGCCATCA TCCCACACAT CAGCACCAAG ACCATAGACA 901 GCTGGATGAG CATCATGGTG CCCAAGAGGG TGCAGGTGAT CCTGCCCAAG TTCACAGCTG 961 TAGCACAAAC AGATTTGAAG GAGCCGCTGA AAGTTCTTGG CATTACTGAC ATGTTTGATT 1021 CATCAAAGGC AAATTTTGCA AAAATAACAA GGTCAGAAAA CCTCCATGTT TCTCATATCT 1081 TGCAAAAAGC AAAAATTGAA GTCAGTGAAG ATGGAACCAA AGCTTCAGCA GCAACAACTG 1141 CAATTCTCAT TGCAAGATCA TCGCCTCCCT GGTTTATAGT AGACAGACCT TTTCTGTTTT 1201 TCATCCGACA TAATCCTACA GGTGCTGTGT TATTCATGGG GCAGATAAAC AAACCCTGCC 1261 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1321 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1381 ATATATCTTG TGGAAAGGAC GAATCCTGAG CATCCAATCC CGTCTCACGC GTTAAGTCga 1441 caatcaacct ctggattaca aaatttgtga aagatt