Transcript: Human XM_005246641.2

PREDICTED: Homo sapiens serpin family E member 2 (SERPINE2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SERPINE2 (5270)
Length:
2243
CDS:
297..1529

Additional Resources:

NCBI RefSeq record:
XM_005246641.2
NBCI Gene record:
SERPINE2 (5270)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005246641.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052315 CCTCGTCAACGCAGTGTATTT pLKO.1 878 CDS 100% 13.200 18.480 N SERPINE2 n/a
2 TRCN0000290150 CCTCGTCAACGCAGTGTATTT pLKO_005 878 CDS 100% 13.200 18.480 N SERPINE2 n/a
3 TRCN0000052313 CGGCGTAAATGGAGTTGGTAA pLKO.1 581 CDS 100% 4.950 6.930 N SERPINE2 n/a
4 TRCN0000290223 CGGCGTAAATGGAGTTGGTAA pLKO_005 581 CDS 100% 4.950 6.930 N SERPINE2 n/a
5 TRCN0000052316 GAACACAAAGAAACGCACTTT pLKO.1 932 CDS 100% 4.950 3.465 N SERPINE2 n/a
6 TRCN0000290224 GAACACAAAGAAACGCACTTT pLKO_005 932 CDS 100% 4.950 3.465 N SERPINE2 n/a
7 TRCN0000052317 GTTTATAGTAGACAGACCTTT pLKO.1 1442 CDS 100% 4.950 3.465 N SERPINE2 n/a
8 TRCN0000290152 GTTTATAGTAGACAGACCTTT pLKO_005 1442 CDS 100% 4.950 3.465 N SERPINE2 n/a
9 TRCN0000052314 GTTTGATTCATCAAAGGCAAA pLKO.1 1280 CDS 100% 0.405 0.284 N SERPINE2 n/a
10 TRCN0000290226 GTTTGATTCATCAAAGGCAAA pLKO_005 1280 CDS 100% 0.405 0.284 N SERPINE2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005246641.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01193 pDONR223 100% 97% 97% None 1_36del n/a
2 ccsbBroad304_01193 pLX_304 0% 97% 97% V5 1_36del n/a
3 TRCN0000470631 ACCCAATCCCCATTTAAAGCCACT pLX_317 38.7% 97% 97% V5 1_36del n/a
4 ccsbBroadEn_01194 pDONR223 100% 96.8% 96.5% None 1_36del;1022_1024delCAG n/a
5 ccsbBroad304_01194 pLX_304 0% 96.8% 96.5% V5 1_36del;1022_1024delCAG n/a
6 TRCN0000471223 ATCCTGAGCATCCAATCCCGTCTC pLX_317 42.3% 96.8% 96.5% V5 1_36del;1022_1024delCAG n/a
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