Construct: ORF TRCN0000471227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015423.1_s317c1
- Derived from:
- ccsbBroadEn_00187
- DNA Barcode:
- CAGAGCTCGTCCAAAATCATGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN5 (726)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 726 | CAPN5 | calpain 5 | NM_004055.5 | 100% | 100% | |
| 2 | human | 726 | CAPN5 | calpain 5 | XM_017018223.2 | 100% | 100% | |
| 3 | human | 726 | CAPN5 | calpain 5 | XM_011545225.1 | 94.1% | 94.1% | 1_120del |
| 4 | mouse | 12337 | Capn5 | calpain 5 | NM_001301250.1 | 88% | 92.1% | (many diffs) |
| 5 | mouse | 12337 | Capn5 | calpain 5 | NM_007602.4 | 88% | 92.1% | (many diffs) |
| 6 | mouse | 12337 | Capn5 | calpain 5 | XM_006507275.3 | 86.2% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctcgtgtgtg aagccctatg aggaccagaa ctactcagcc ctgaggcggg 121 actgccggcg caggaaggtg ctcttcgagg accccctctt ccccgccact gacgactcac 181 tctactataa gggcacgccg gggcccgccg tcaggtggaa gcgacccaag ggcatctgcg 241 aggacccccg cctctttgtg gatggcatca gctcccacga cctgcaccag ggccaggtgg 301 gcaactgctg gtttgtggca gcctgctcgt cacttgcctc ccgggagtcg ctgtggcaaa 361 aggtcatccc agactggaag gagcaggaat gggaccccga aaagcccaac gcctacgcgg 421 gcatcttcca cttccacttc tggcgcttcg gggaatgggt ggacgtggtc atcgatgacc 481 ggctgcccac agtcaacaac cagctcatct actgccactc caactcccgc aatgagtttt 541 ggtgcgccct agtggagaag gcctatgcca aactggcagg ctgttaccag gccctggatg 601 gaggcaacac agcagacgca ctggtggact tcacgggtgg tgtttctgag cccatcgacc 661 tgaccgaggg tgactttgcc aacgatgaga ctaagaggaa ccagctcttt gagcgcatgt 721 taaaggtgca cagccggggc ggcctcatca gtgcctccat caaggcagtg acagcagctg 781 acatggaggc ccgcctggcg tgcggcctgg taaagggcca cgcatacgcc gtcactgatg 841 tgcgcaaggt gcgcctgggc cacggcctac tggccttctt caagtcagag aagttggaca 901 tgatccgcct gcgcaacccc tggggcgagc gggagtggaa cgggccctgg agtgacacct 961 cggaggagtg gcagaaagtg agcaagagtg agcgggagaa gatgggtgtg accgtgcagg 1021 acgacggtga gttctggatg accttcgagg acgtgtgccg gtacttcacg gacatcatca 1081 agtgccgcgt gatcaacaca tcccacctga gcatccacaa gacgtgggag gaggcccggc 1141 tgcatggcgc ctggacgctg catgaggacc cgcgacagaa ccgcggtggc ggctgcatca 1201 accacaagga caccttcttc cagaacccac agtacatctt cgaagtcaag aagccagaag 1261 atgaagtcct gatctgcatc cagcagcggc caaagcggtc tacgcgccgg gagggcaagg 1321 gtgagaacct ggccattggc tttgacatct acaaggtgga ggagaaccgc cagtaccgca 1381 tgcacagcct gcagcacaag gccgccagct ccatctacat caactcacgc agcgtcttcc 1441 tgcgcaccga ccagcccgag ggccgctatg tcatcatccc cacaaccttc gagccaggcc 1501 acactggcga gttcctgcTC CGAGTCTTCA CTGATGTGCC CTCCAACTGC CGGGAGCTGC 1561 GCCTGGATGA GCCCCCACAC ACCTGCTGGA GCTCCCTCTG TGGCTACCCC CAGCTGGTGA 1621 CCCAGGTACA TGTCCTGGGA GCTGCTGGCC TCAAGGACTC CCCAACAGGG GCTAACTCTT 1681 ATGTGATCAT CAAGTGTGAG GGAGACAAAG TCCGCTCGGC TGTGCAGAAG GGCACCTCCA 1741 CACCAGAGTA CAATGTGAAA GGCATCTTCT ACCGCAAGAA GCTGAGCCAG CCCATCACTG 1801 TACAGGTCTG GAACCACCGA GTGCTGAAGG ATGAATTTCT GGGCCAGGTG CACCTAAAGG 1861 CTGACCCGGA CAACCTCCAG GCCCTGCATA CCCTCCACCT CCGGGACCGA AATAGCCGGC 1921 AGCCCAGCAA CCTGCCAGGC ACTGTGGCCG TGCACATTCT CAGCAGCACC TCCCTCATGG 1981 CTGTCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGAGCTCG TCCAAAATCA TGAACACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt