Construct: ORF TRCN0000471250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015653.1_s317c1
- Derived from:
- ccsbBroadEn_02218
- DNA Barcode:
- CTATGAAATCCTCCGTGAGACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IKBKE (9641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193321.2 | 100% | 100% | |
| 2 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_017002868.1 | 94.1% | 94.1% | 0_1ins111 |
| 3 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_014002.4 | 88.1% | 88.1% | 1_255del |
| 4 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_005273356.2 | 88.1% | 88.1% | 1_255del |
| 5 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193322.2 | 79.8% | 78.6% | 1_255del;1932_1933ins113;1971_1972ins64 |
| 6 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XR_922002.2 | 57.7% | 1_607del;1949_1950insAGGTACATGAGGACAGA;2484_3231del | |
| 7 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529762.3 | 56.2% | 57.4% | (many diffs) |
| 8 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529763.3 | 48.9% | 49% | (many diffs) |
| 9 | mouse | 56489 | Ikbke | inhibitor of kappaB kinase ... | XM_006529764.3 | 47.8% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1962
- ORF length:
- 1893
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtactgc tccagtggga gcctgctgag tgtgctggag agccctgaga 121 atgcctttgg gctgcctgag gatgagttcc tggtggtgct gcgctgtgtg gtggccggca 181 tgaaccacct gcgggagaac ggcattgtgc atcgcgacat caagccgggg aacatcatgc 241 gcctcgtagg ggaggagggg cagagcatct acaagctgac agacttcggc gctgcccggg 301 agctggatga tgatgagaag ttcgtctcgg tctatgggac tgaggagtac ctgcatcccg 361 acatgtatga gcgggcggtg cttcgaaagc cccagcaaaa agcgttcggg gtgactgtgg 421 atctctggag cattggagtg accttgtacc atgcagccac tggcagcctg cccttcatcc 481 cctttggtgg gccacggcgg aacaaggaga tcatgtaccg gatcaccacg gagaagccgg 541 ctggggccat tgcaggtgcc cagaggcggg agaacgggcc cctggagtgg agctacaccc 601 tccccatcac ctgccagctg tcactggggc tgcagagcca gctggtgccc atcctggcca 661 acatcctgga ggtggagcag gccaagtgct ggggcttcga ccagttcttt gcggagacca 721 gtgacatcct gcagcgagtt gtcgtccatg tcttctccct gtcccaggca gtcctgcacc 781 acatctatat ccatgcccac aacacgatag ccattttcca ggaggccgtg cacaagcaga 841 ccagtgtggc cccccgacac caggagtacc tctttgaggg tcacctctgt gtcctcgagc 901 ccagcgtctc agcacagcac atcgcccaca cgacggcaag cagccccctg accctcttca 961 gcacagccat ccctaagggg ctggccttca gggaccctgc tctggacgtc cccaagttcg 1021 tccccaaagt ggacctgcag gcggattaca acactgccaa gggcgtgttg ggcgccggct 1081 accaggccct gcggctggca cgggccctgc tggatgggca ggagctaatg tttcgggggc 1141 tgcactgggt catggaggtg ctccaggcca catgcagacg gactctggaa gtggcaagga 1201 catccctcct ctacctcagc agcagcctgg gaactgagag gttcagcagc gtggctggaa 1261 cgcctgagat ccaggaactg aaggcggctg cagaactgag gtccaggctg cggactctag 1321 cggaggtcct ctccagatgc tcccaaaata tcacggagac ccaggagagc ctgagcagcc 1381 tgaaccggga gctggtgaag agccgggatc aggtacatga ggacagaagc atccagcaga 1441 ttcagtgctg tttggacaag atgaacttca tctacaaaca gttcaagaag tctaggatga 1501 ggccagggct tggctacaac gaggagcaga ttcacaagct ggataaggtg aatttcagtc 1561 atttagccaa aagactcctg caggtgttcc aggaggagtg cgtgcagaag tatcaagcgt 1621 ccTTAGTCAC ACACGGCAAG AGGATGAGGG TGGTGCACGA GACCAGGAAC CACCTGCGCC 1681 TGGTTGGCTG TTCTGTGGCT GCCTGTAACA CAGAAGCCCA GGGGGTCCAG GAGAGTCTCA 1741 GCAAGCTCCT GGAAGAGCTA TCTCACCAGC TCCTTCAGGA CCGAGCAAAG GGGGCTCAGG 1801 CCTCGCCGCC TCCCATAGCT CCTTACCCCA GCCCTACACG AAAGGACCTG CTTCTCCACA 1861 TGCAAGAGCT CTGCGAGGGG ATGAAGCTGC TGGCATCTGA CCTCCTGGAC AACAACCGCA 1921 TCATCGAACG GCTAAATAGA GTCCCAGCAC CTCCTGATGT CTTGCCAACT TTCTTGTACA 1981 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2041 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2101 AGGACGACTA TGAAATCCTC CGTGAGACTT AACGCGTTAA GTCgacaatc aacctctgga 2161 ttacaaaatt tgtgaaagat t