Construct: ORF TRCN0000471261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009748.1_s317c1
- Derived from:
- ccsbBroadEn_04103
- DNA Barcode:
- CTTACCCAGAGGAGATCAAATCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX40 (79665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79665 | DHX40 | DEAH-box helicase 40 | NM_024612.5 | 100% | 100% | |
| 2 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_006722083.3 | 96.5% | 96.5% | 1341_1342ins81 |
| 3 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_017025080.2 | 92.8% | 92.8% | 110_111ins168 |
| 4 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_011525253.3 | 92.1% | 92.1% | 1160_1161ins183 |
| 5 | human | 79665 | DHX40 | DEAH-box helicase 40 | NM_001166301.2 | 90.1% | 90.1% | 195_196ins231 |
| 6 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_011525254.2 | 87.2% | 87.2% | 0_1ins297 |
| 7 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_017025081.1 | 83.8% | 83.8% | 0_1ins297;1044_1045ins81 |
| 8 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_017025082.1 | 82.1% | 81.7% | 0_1ins409;4_5insTTGC;7_7delCinsTTCTA |
| 9 | human | 79665 | DHX40 | DEAH-box helicase 40 | XM_017025083.1 | 82.1% | 81.7% | 0_1ins409;4_5insTTGC;7_7delCinsTTCTA |
| 10 | mouse | 67487 | Dhx40 | DEAH (Asp-Glu-Ala-His) box ... | NM_026191.2 | 91.5% | 97.5% | (many diffs) |
| 11 | mouse | 67487 | Dhx40 | DEAH (Asp-Glu-Ala-His) box ... | XM_017314718.1 | 60.7% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2403
- ORF length:
- 2337
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccggtttccc gcagtcgcgg gcagggcgcc aaggcggcag gaggagggtg 121 agcggtcaag agacctccag gaagagcggc tctcggctgt ttgcatcgcc gatagagaag 181 agaaaggatg cacgtcccag gagggaggaa ctactccaac ttttcctatt cagaaacaaa 241 gaaaaaagat tattcaagct gtgagggaca attcattcct tattgttact ggaaatacag 301 gaagtggtaa aacaactcaa ctcccaaaat atctatatga agcagggttt tcacaacatg 361 gtatgattgg tgtaactcaa ccacgaaaag tagctgctat atcagttgct cagagagtag 421 ctgaagaaat gaaatgcact ttgggatcca aagtaggata ccaagttcgt tttgatgatt 481 gcagttctaa ggagacagca atcaaatata tgactgatgg atgtttactg aaacatattc 541 tgggagaccc aaatcttacc aaattcagtg tcattatttt ggatgaagcc catgaaagaa 601 ctctaactac agatatctta tttggtttat tgaagaagct atttcaggag aagtctccta 661 ataggaagga gcatttaaaa gtggtggtaa tgtcagcaac tatggaatta gccaagctct 721 ctgcattctt tggaaattgt ccaatatttg atatacctgg aaggctttat ccagtcagag 781 agaaattctg caatttgatt ggtccacgag acagagaaaa tactgcgtat attcaagcga 841 ttgtgaaagt caccatggat atccatttga atgaaatggc tggagacatc ttggtttttc 901 tgactggcca gtttgaaata gaaaaaagtt gtgagttact ttttcagatg gcagagtctg 961 ttgattatga ttatgatgtt caagatacca ccctcgatgg cttgttaata ttgccgtgtt 1021 atggatcaat gacaacagat caacagagga ggatattttt gccaccacca cctggaatta 1081 gaaaatgtgt catatccacc aatatttctg caacgtcttt gacaatagat ggaatcagat 1141 atgtggtaga tggtggcttc gtgaagcagt taaatcacaa ccccagatta gggttggaca 1201 tcctggaggt ggttccaatt tcaaagagcg aggcattaca gcgaagtggc cgagctggca 1261 ggacttcttc aggaaaatgc tttcggatct atagtaaaga tttttggaac cagtgtatgc 1321 ctgaccatgt gatccctgaa attaagagaa ctagtttgac atctgtagtt ctgaccttaa 1381 agtgccttgc catacacgat gtcataaggt ttccctattt ggatccacct aatgagagac 1441 ttattttaga agctcttaaa caactttacc agtgtgatgc tattgacagg agtggccatg 1501 tcaccagatt gggtttgtct atggtggagt ttcctttgcc tccacatctg acatgtgcag 1561 taataaaagc tgcttccctg gattgtgaag atctactact tccaatagca gcaatgttgt 1621 ctgtggaaaa cgtcttcatt agacctgttg atccagagta ccagaaggaa gcagaacaga 1681 gacatcgaga attggcagct aaagctggag gatttaatga ctttgcaact ttagctgtca 1741 tctttgaaca atgcaaatca agtggagctc cagcttcatg gtgccaaaaa cactggattc 1801 attggaggtg cttattttct gcatttcgtg tggaagctca acttcgagaa ctaatcagga 1861 agcttaaaca gcaaagtgat ttcccaaaag agacctttga aggccctaaa catgaagtac 1921 tacgaagatg tctttgtgcg ggctatttca aaaatgtagc tcgaagatct gttgggagaa 1981 cgttttgcac aatggatggt cgtggaagcc cagttcacat tcatccttcc tcagcacttc 2041 atgaacagga aaccaaactt gaatggatca tttttcatga ggtattggtt acCACCAAAG 2101 TCTACGCAAG AATTGTATGC CCAATCCGTT ATGAATGGGT AAGAGACTTG TTACCCAAGT 2161 TGCATGAATT TAATGCACAT GATTTGAGCA GTGTGGCCCG ACGTGAAGTG AGAGAAGATG 2221 CAAGAAGGAG ATGGACAAAT AAGGAAAATG TAAAGCAGCT AAAGGATGGA ATATCGAAAG 2281 ACGTCTTAAA GAAAATGCAA AGAAGAAATG ATGACAAATC CATATCTGAT GCACGGGCTC 2341 GTTTCCTTGA GAGAAAGCAG CAGAGGACCC AGGACCACAG TGACACACGA AAGGAAACAG 2401 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2461 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2521 GGCTTTATAT ATCTTGTGGA AAGGACGACT TACCCAGAGG AGATCAAATC AAACGCGTTA 2581 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt