Construct: ORF TRCN0000471262
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001445.2_s317c1
- Derived from:
- ccsbBroadEn_09318
- DNA Barcode:
- TTAATACTGAATGATTGGCCACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLXNA4 (91584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471262
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91584 | PLXNA4 | plexin A4 | NM_181775.3 | 99.8% | 99.8% | 1395G>C;1424A>C |
| 2 | human | 91584 | PLXNA4 | plexin A4 | NM_001105543.2 | 91.2% | 87.1% | (many diffs) |
| 3 | human | 91584 | PLXNA4 | plexin A4 | XR_927546.2 | 34.9% | (many diffs) | |
| 4 | human | 91584 | PLXNA4 | plexin A4 | XM_011516676.2 | 34.7% | 32.5% | (many diffs) |
| 5 | human | 91584 | PLXNA4 | plexin A4 | XM_017012779.1 | 27.3% | 25.6% | (many diffs) |
| 6 | human | 91584 | PLXNA4 | plexin A4 | NM_020911.1 | 26.4% | 24.7% | (many diffs) |
| 7 | human | 91584 | PLXNA4 | plexin A4 | XM_005250686.5 | 26.4% | 24.7% | (many diffs) |
| 8 | human | 91584 | PLXNA4 | plexin A4 | XM_006716171.4 | 26.4% | 24.7% | (many diffs) |
| 9 | mouse | 243743 | Plxna4 | plexin A4 | NM_175750.3 | 23.6% | 23.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agccatgccc tggaactgga cctgccttct ctcccacctc ctcatggtgg 121 gcatgggctc ctccactttg ctcacccggc agccagcccc gctgtcccag aagcagcggt 181 catttgtcac attccgagga gagcccgccg agggtttcaa tcacctggtg gtggatgaga 241 ggacaggaca catttacttg ggggccgtca atcggattta caagctctcc agcgacctga 301 aggtcttggt gacgcatgag acagggccgg acgaggacaa ccccaagtgt tacccacccc 361 gcatcgtcca gacctgcaat gagcccctga ccaccaccaa caatgtcaac aagatgctcc 421 tcatagacta caaggagaac aggctgattg cctgtgggag cctgtaccaa ggcatctgca 481 agctgctgag gctggaggac ctcttcaagc tgggggagcc ttatcataag aaggagcact 541 atctgtcagg tgtcaacgag agcggctcag tctttggagt gatcgtctcc tacagcaacc 601 tggatgacaa gctgttcatt gccacggcag tggatgggaa gcccgagtat tttcccacca 661 tctccagccg gaaactgacc aagaactctg aggcggatgg catgttcgcg tacgtcttcc 721 atgatgagtt cgtggcctcg atgattaaga tcccttcgga caccttcacc atcatccctg 781 actttgatat ctactatgtc tatggtttta gcagtggcaa ctttgtctac tttttgaccc 841 tccaacctga gatggtgtct ccaccaggct ccaccaccaa ggagcaggtg tatacatcca 901 agctcgtgag gctttgcaag gaggacacag ccttcaactc ctatgtagag gtgcccattg 961 gctgtgagcg cagtggggtg gagtaccgcc tgctgcaggc tgcctacctg tccaaagcgg 1021 gggccgtgct tggcaggacc cttggagtcc atccagatga tgacctgctc ttcaccgtct 1081 tctccaaggg ccagaagcgg aaaatgaaat ccctggatga gtcggccctg tgcatcttca 1141 tcttgaagca gataaatgac cgcattaagg agcggctgca gtcttgttac cggggcgagg 1201 gcacgctgga cctggcctgg ctcaaggtga aggacaTCCC CTGCAGCAGT GCGCTCTTAA 1261 CCATTGACGA TAACTTCTGT GGCCTGGACA TGAATGCTCC CCTGGGAGTG TCCGACATGG 1321 TGCGTGGAAT TCCCGTCTTC ACGGAGGACA GGGACCGCAT GACGTCTGTC ATCGCATATG 1381 TCTACAAGAA CCACTCTCTG GCCTTTGTGG GCACCAAAAG TGGCAAGCTG AAGAAGTCCT 1441 TCGGCACAGG TCCACAAGGC GGAATCACCC AGGAGTGGAT TGGAGTGGCG GGAGACCCAC 1501 CTGGGGCGAA CATTGCTTCT CAGGAACAGA TGCTGTGTGT GTATCTTCAG TGCTCAAGTC 1561 ACAAAGCAAT ATCAGATCAG AGAGTCCAGC CTCTGCTTTG TTGCTTCCTA AATGTACCTG 1621 GGAATTCTTC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTA ATACTGAATG ATTGGCCACG 1801 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t