Transcript: Human NM_020911.1

Homo sapiens plexin A4 (PLXNA4), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
PLXNA4 (91584)
Length:
13061
CDS:
230..5914

Additional Resources:

NCBI RefSeq record:
NM_020911.1
NBCI Gene record:
PLXNA4 (91584)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001162265 GCGGTATGGACTCTCCACGC pXPR_003 AGG 1996 35% 9 0.9279 PLXNA4 PLXNA4 76879
2 BRDN0001145875 TGTATACATCCAAGCTCGTG pXPR_003 AGG 840 15% 2 0.2682 PLXNA4 PLXNA4 76880
3 BRDN0001148179 ACATGATGTAGAGTTGCTCG pXPR_003 TGG 1475 26% 4 0.2451 PLXNA4 PLXNA4 76881
4 BRDN0001162300 CCTGAATGCCGGAAGCAACG pXPR_003 TGG 2950 52% 15 0.1614 PLXNA4 PLXNA4 76878
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020911.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359758 GTTCGTGGCCTCGATGATTAA pLKO_005 892 CDS 100% 13.200 18.480 N PLXNA4 n/a
2 TRCN0000078684 GCTCTTAACCATTGACGATAA pLKO.1 1417 CDS 100% 10.800 15.120 N PLXNA4 n/a
3 TRCN0000078685 GCAGCGGTCATTTGTCACATT pLKO.1 337 CDS 100% 4.950 6.930 N PLXNA4 n/a
4 TRCN0000078686 GCGCTCTTAACCATTGACGAT pLKO.1 1415 CDS 100% 2.640 3.696 N PLXNA4 n/a
5 TRCN0000078683 GCAGATAAATGACCGCATTAA pLKO.1 1312 CDS 100% 13.200 9.240 N PLXNA4 n/a
6 TRCN0000359686 TGGATGGGAAGCCCGAGTATT pLKO_005 795 CDS 100% 13.200 9.240 N PLXNA4 n/a
7 TRCN0000359757 CTCTTAACCATTGACGATAAC pLKO_005 1418 CDS 100% 10.800 7.560 N PLXNA4 n/a
8 TRCN0000078687 CCTGACTTTGATATCTACTAT pLKO.1 941 CDS 100% 5.625 3.938 N PLXNA4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020911.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09318 pDONR223 100% 26.4% 24.7% None (many diffs) n/a
2 ccsbBroad304_09318 pLX_304 0% 26.4% 24.7% V5 (many diffs) n/a
3 TRCN0000471262 TTAATACTGAATGATTGGCCACGG pLX_317 25.5% 26.4% 24.7% V5 (many diffs) n/a
4 ccsbBroadEn_15213 pDONR223 0% 26.4% 24.7% None (many diffs) n/a
5 ccsbBroad304_15213 pLX_304 0% 26.4% 24.7% V5 (many diffs) n/a
6 TRCN0000479825 ATTTGACATTCATAACGAACGTTT pLX_317 22.5% 26.4% 24.7% V5 (many diffs) n/a
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