Construct: ORF TRCN0000471305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016726.1_s317c1
- Derived from:
- ccsbBroadEn_10771
- DNA Barcode:
- ATTAACCCGCCAATCATTCTCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCTN1 (1639)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001135041.2 | 17.3% | 17.2% | 1_2824delinsA |
| 2 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_023019.3 | 17.2% | 17.2% | 1_2839delinsA |
| 3 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001190836.1 | 15.9% | 15.9% | 1_3115delinsA |
| 4 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001135040.2 | 15.7% | 15.7% | 1_3166delinsA |
| 5 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001190837.1 | 15.5% | 15.4% | 1_3220delinsA |
| 6 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_004082.4 | 15.4% | 15.4% | 1_3241delinsA |
| 7 | human | 1639 | DCTN1 | dynactin subunit 1 | NR_033935.1 | 13.2% | 1_3510delinsA;4104_4469del | |
| 8 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505495.4 | 15.5% | 16.3% | (many diffs) |
| 9 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001347310.1 | 15.4% | 16.3% | (many diffs) |
| 10 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001198867.1 | 14.2% | 15% | (many diffs) |
| 11 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255137.1 | 14.2% | 15% | (many diffs) |
| 12 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505493.2 | 14.2% | 15% | (many diffs) |
| 13 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505494.1 | 14.2% | 15% | (many diffs) |
| 14 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255136.1 | 14.2% | 15% | (many diffs) |
| 15 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505492.2 | 14.1% | 14.9% | (many diffs) |
| 16 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505491.4 | 14.1% | 14.8% | (many diffs) |
| 17 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505490.2 | 14% | 14.8% | (many diffs) |
| 18 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505489.3 | 14% | 14.8% | (many diffs) |
| 19 | mouse | 13191 | Dctn1 | dynactin 1 | XM_017321379.2 | 14% | 14.8% | (many diffs) |
| 20 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001198866.1 | 14% | 14.7% | (many diffs) |
| 21 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505488.3 | 14% | 14.7% | (many diffs) |
| 22 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255135.1 | 14% | 14.7% | (many diffs) |
| 23 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505487.3 | 13.9% | 14.7% | (many diffs) |
| 24 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505486.4 | 13.8% | 14.6% | (many diffs) |
| 25 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505485.2 | 13.8% | 14.6% | (many diffs) |
| 26 | mouse | 13191 | Dctn1 | dynactin 1 | NM_007835.2 | 13.8% | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggcccaggg ctggtgaagg actcaccact gctgcttcag cagatctctg 121 ccatgaggct gcacatctcc cagctccagc atgagaacag catcctcaag ggagcccaga 181 tgaaggcatc cttggcatcc cTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC 241 CTGGCAGTGA GTTACCAGCT GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT 301 TGAATCAATT GAGCACACAC ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA 361 AGAGCCCGTC GGCCCAACTT ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG 421 TCGAGAAGCT CAAGGATGAG GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG 481 TACCCACTGA CTTTGCCACC TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC 541 AGGATGACAC AGTCTACATG GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC 601 GACACCGGCT GGTGCTGACC CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT 661 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGAATTAA CCCGCCAATC ATTCTCCCTA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt