Construct: ORF TRCN0000471305

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016726.1_s317c1
Derived from:
ccsbBroadEn_10771
DNA Barcode:
ATTAACCCGCCAATCATTCTCCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCTN1 (1639)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471305
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1639 DCTN1 dynactin subunit 1 NM_001135041.2 17.3% 17.2% 1_2824delinsA
2 human 1639 DCTN1 dynactin subunit 1 NM_023019.3 17.2% 17.2% 1_2839delinsA
3 human 1639 DCTN1 dynactin subunit 1 NM_001190836.1 15.9% 15.9% 1_3115delinsA
4 human 1639 DCTN1 dynactin subunit 1 NM_001135040.2 15.7% 15.7% 1_3166delinsA
5 human 1639 DCTN1 dynactin subunit 1 NM_001190837.1 15.5% 15.4% 1_3220delinsA
6 human 1639 DCTN1 dynactin subunit 1 NM_004082.4 15.4% 15.4% 1_3241delinsA
7 human 1639 DCTN1 dynactin subunit 1 NR_033935.1 13.2% 1_3510delinsA;4104_4469del
8 mouse 13191 Dctn1 dynactin 1 XM_006505495.4 15.5% 16.3% (many diffs)
9 mouse 13191 Dctn1 dynactin 1 NM_001347310.1 15.4% 16.3% (many diffs)
10 mouse 13191 Dctn1 dynactin 1 NM_001198867.1 14.2% 15% (many diffs)
11 mouse 13191 Dctn1 dynactin 1 XM_030255137.1 14.2% 15% (many diffs)
12 mouse 13191 Dctn1 dynactin 1 XM_006505493.2 14.2% 15% (many diffs)
13 mouse 13191 Dctn1 dynactin 1 XM_006505494.1 14.2% 15% (many diffs)
14 mouse 13191 Dctn1 dynactin 1 XM_030255136.1 14.2% 15% (many diffs)
15 mouse 13191 Dctn1 dynactin 1 XM_006505492.2 14.1% 14.9% (many diffs)
16 mouse 13191 Dctn1 dynactin 1 XM_006505491.4 14.1% 14.8% (many diffs)
17 mouse 13191 Dctn1 dynactin 1 XM_006505490.2 14% 14.8% (many diffs)
18 mouse 13191 Dctn1 dynactin 1 XM_006505489.3 14% 14.8% (many diffs)
19 mouse 13191 Dctn1 dynactin 1 XM_017321379.2 14% 14.8% (many diffs)
20 mouse 13191 Dctn1 dynactin 1 NM_001198866.1 14% 14.7% (many diffs)
21 mouse 13191 Dctn1 dynactin 1 XM_006505488.3 14% 14.7% (many diffs)
22 mouse 13191 Dctn1 dynactin 1 XM_030255135.1 14% 14.7% (many diffs)
23 mouse 13191 Dctn1 dynactin 1 XM_006505487.3 13.9% 14.7% (many diffs)
24 mouse 13191 Dctn1 dynactin 1 XM_006505486.4 13.8% 14.6% (many diffs)
25 mouse 13191 Dctn1 dynactin 1 XM_006505485.2 13.8% 14.6% (many diffs)
26 mouse 13191 Dctn1 dynactin 1 NM_007835.2 13.8% 14.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
660
ORF length:
594
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc aggcccaggg ctggtgaagg actcaccact gctgcttcag cagatctctg
121ccatgaggct gcacatctcc cagctccagc atgagaacag catcctcaag ggagcccaga
181tgaaggcatc cttggcatcc cTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC
241CTGGCAGTGA GTTACCAGCT GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT
301TGAATCAATT GAGCACACAC ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA
361AGAGCCCGTC GGCCCAACTT ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG
421TCGAGAAGCT CAAGGATGAG GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG
481TACCCACTGA CTTTGCCACC TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC
541AGGATGACAC AGTCTACATG GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC
601GACACCGGCT GGTGCTGACC CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT
661ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
721GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
781TTTATATATC TTGTGGAAAG GACGAATTAA CCCGCCAATC ATTCTCCCTA CGCGTTAAGT
841Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)