Transcript: Human NR_033935.1

Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 7, non-coding RNA.

Source:
NCBI, updated 2019-03-19
Taxon:
Homo sapiens (human)
Gene:
DCTN1 (1639)
Length:
4469
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_033935.1
NBCI Gene record:
DCTN1 (1639)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_033935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000063970 GCTGGAGACATTGAATCAATT pLKO.1 3734 3UTR 100% 13.200 9.240 N DCTN1 n/a
2 TRCN0000299622 GCTGGAGACATTGAATCAATT pLKO_005 3734 3UTR 100% 13.200 9.240 N DCTN1 n/a
3 TRCN0000063971 CCAGAGACCTTTGACTTCAAA pLKO.1 1849 3UTR 100% 5.625 3.938 N DCTN1 n/a
4 TRCN0000299565 CCAGAGACCTTTGACTTCAAA pLKO_005 1849 3UTR 100% 5.625 3.938 N DCTN1 n/a
5 TRCN0000063969 GCCCAGGAGAAGTTTGAACTA pLKO.1 2077 3UTR 100% 4.950 3.465 N DCTN1 n/a
6 TRCN0000299620 GCCCAGGAGAAGTTTGAACTA pLKO_005 2077 3UTR 100% 4.950 3.465 N DCTN1 n/a
7 TRCN0000063968 GCCCATCTACAGGATGTGAAT pLKO.1 1774 3UTR 100% 4.950 2.970 N DCTN1 n/a
8 TRCN0000299621 GCCCATCTACAGGATGTGAAT pLKO_005 1774 3UTR 100% 4.950 2.970 N DCTN1 n/a
9 TRCN0000063972 GCCATCAAGTACTATCAGCAT pLKO.1 2368 3UTR 100% 2.640 1.584 N DCTN1 n/a
10 TRCN0000299623 GCCATCAAGTACTATCAGCAT pLKO_005 2368 3UTR 100% 2.640 1.584 N DCTN1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_033935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06086 pDONR223 100% 76.1% None (many diffs) n/a
2 ccsbBroad304_06086 pLX_304 0% 76.1% V5 (many diffs) n/a
3 TRCN0000491671 CGGCAGCGCTTACGCACGCAAGAA pLX_317 8% 76.1% V5 (many diffs) n/a
4 ccsbBroadEn_10771 pDONR223 100% 13.2% None 1_3510delinsA;4104_4469del n/a
5 ccsbBroad304_10771 pLX_304 0% 13.2% V5 1_3510delinsA;4104_4469del n/a
6 TRCN0000471305 ATTAACCCGCCAATCATTCTCCCT pLX_317 76.3% 13.2% V5 1_3510delinsA;4104_4469del n/a
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