Construct: ORF TRCN0000471308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001135.1_s317c1
- Derived from:
- ccsbBroadEn_04373
- DNA Barcode:
- CCTAGACTTCTCAAATCAGGACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS25 (84313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84313 | VPS25 | vacuolar protein sorting 25... | NM_032353.4 | 100% | 100% | |
| 2 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | NM_026776.4 | 92.6% | 98.8% | (many diffs) |
| 3 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | NM_001284411.1 | 88.6% | 94.5% | (many diffs) |
| 4 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | NM_001284412.1 | 82.4% | 79.8% | (many diffs) |
| 5 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | NM_001284414.1 | 78% | 77.6% | (many diffs) |
| 6 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | XM_006533504.3 | 67.7% | 67.4% | (many diffs) |
| 7 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | XM_017314585.1 | 44.1% | 44.6% | (many diffs) |
| 8 | mouse | 28084 | Vps25 | vacuolar protein sorting 25 | XM_006533505.3 | 42.4% | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gatgagtttc gagtggccgt ggcagtatcg cttcccaccc ttctttacgt 121 tacaaccgaa tgtggacact cggcagaagc agctggccgc cTGGTGCTCG CTGGTCCTGT 181 CCTTCTGCCG CCTGCACAAA CAGTCCAGCA TGACGGTGAT GGAAGCTCAG GAGAGCCCGC 241 TCTTCAACAA CGTCAAGCTA CAGCGAAAGC TTCCTGTGGA GTCGATCCAG ATTGTATTAG 301 AGGAACTGAG GAAGAAAGGG AACCTCGAGT GGTTGGATAA GAGCAAGTCC AGCTTCCTGA 361 TCATGTGGCG GAGGCCAGAA GAATGGGGGA AACTCATCTA TCAGTGGGTT TCCAGGAGTG 421 GCCAGAACAA CTCCGTCTTT ACCCTGTATG AACTGACTAA TGGGGAAGAC ACAGAGGATG 481 AGGAGTTCCA CGGGCTGGAT GAAGCCACTC TACTGCGGGC TCTGCAGGCC CTACAGCAGG 541 AGCACAAGGC CGAGATCATC ACTGTCAGCG ATGGCCGAGG CGTCAAGTTC TTCTACCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAC CTAGACTTCT CAAATCAGGA CACACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att