Transcript: Mouse NM_001284411.1

Mus musculus vacuolar protein sorting 25 (Vps25), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Vps25 (28084)
Length:
1143
CDS:
49..603

Additional Resources:

NCBI RefSeq record:
NM_001284411.1
NBCI Gene record:
Vps25 (28084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001284411.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000173175 CTTCTTTACGTTACAGCCGAA pLKO.1 93 CDS 100% 2.160 3.024 N Vps25 n/a
2 TRCN0000175919 GTTTCCCACATAACCCTTTAT pLKO.1 744 3UTR 100% 13.200 10.560 N Vps25 n/a
3 TRCN0000336112 GTTTCCCACATAACCCTTTAT pLKO_005 744 3UTR 100% 13.200 10.560 N Vps25 n/a
4 TRCN0000175819 GCCAGAATAACTCTGTGTTTA pLKO.1 428 CDS 100% 13.200 9.240 N Vps25 n/a
5 TRCN0000336110 GCCAGAATAACTCTGTGTTTA pLKO_005 428 CDS 100% 13.200 9.240 N Vps25 n/a
6 TRCN0000336171 TCGAGTGGTTGGATAAGAATA pLKO_005 332 CDS 100% 13.200 9.240 N Vps25 n/a
7 TRCN0000173433 CTTAGGGATGTTTCCCACATA pLKO.1 735 3UTR 100% 4.950 3.465 N Vps25 n/a
8 TRCN0000336111 CTTAGGGATGTTTCCCACATA pLKO_005 735 3UTR 100% 4.950 3.465 N Vps25 n/a
9 TRCN0000381965 ACAAGGCTGAGATTATCACTG pLKO_005 551 CDS 100% 4.050 2.835 N Vps25 n/a
10 TRCN0000382481 GTCTAGCTTCCTGATCATGTG pLKO_005 354 CDS 100% 4.050 2.835 N Vps25 n/a
11 TRCN0000382455 ACTCATTTACCAATGGGTTTC pLKO_005 399 CDS 100% 6.000 3.600 N Vps25 n/a
12 TRCN0000194225 GAAACTCATTTACCAATGGGT pLKO.1 396 CDS 100% 0.750 0.450 N Vps25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001284411.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04373 pDONR223 100% 88.6% 94.5% None (many diffs) n/a
2 ccsbBroad304_04373 pLX_304 0% 88.6% 94.5% V5 (many diffs) n/a
3 TRCN0000471308 CCTAGACTTCTCAAATCAGGACAC pLX_317 80.8% 88.6% 94.5% V5 (many diffs) n/a
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