Construct: ORF TRCN0000471315
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013721.1_s317c1
- Derived from:
- ccsbBroadEn_07829
- DNA Barcode:
- CCGAACCAGGACAAAGAAAGTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOSC7 (23016)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471315
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23016 | EXOSC7 | exosome component 7 | NM_015004.4 | 99.8% | 99.6% | 820G>C |
| 2 | human | 23016 | EXOSC7 | exosome component 7 | XM_017005930.2 | 71.5% | 70.4% | (many diffs) |
| 3 | human | 23016 | EXOSC7 | exosome component 7 | XR_940394.3 | 70.8% | 1_27del;847G>C;901_1230del | |
| 4 | human | 23016 | EXOSC7 | exosome component 7 | XR_245104.4 | 60.4% | 1_27del;847G>C;901_1443del | |
| 5 | human | 23016 | EXOSC7 | exosome component 7 | XR_001740061.1 | 55% | (many diffs) | |
| 6 | human | 23016 | EXOSC7 | exosome component 7 | XR_001740062.1 | 54.1% | (many diffs) | |
| 7 | human | 23016 | EXOSC7 | exosome component 7 | NR_023353.1 | 46.7% | (many diffs) | |
| 8 | mouse | 66446 | Exosc7 | exosome component 7 | NM_001081188.1 | 89.9% | 95.8% | (many diffs) |
| 9 | mouse | 66446 | Exosc7 | exosome component 7 | XM_006512207.3 | 65.1% | 66.6% | (many diffs) |
| 10 | mouse | 66446 | Exosc7 | exosome component 7 | XM_006512208.1 | 50.9% | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccgtgacg ctgagcgagg cggagaaggt gtacatcgtg catggcgtcc 121 aggaagacct ccgtgtggat ggccgtggct gtgaggacta ccgatgtgtc gaagtggaaa 181 ctgatgtggt gtccaacact agtgggtccg ccagggtcaa gctgggtcac acagacatct 241 tggtgggagt gaaagcagaa atggggacgc cgaagctgga gaaaccaaat gaaggctact 301 tggagttctt tgttgactgt tcagccagtg ctacccctga atttgaaggt agaggaggtg 361 atgaccttgg caccgagatc gctaacaccc tctatcggat atttaacaat aaaagcagtg 421 tcgacttaaa gaccctctgc attagtcctc gggagcactg ctgggttctc tatgtggatg 481 tgctgcttct ggaatgtggt ggaaatttgt ttgatgccat ttccattgct gtaaaggctg 541 ctctcttcaa tacaaggata ccaagggttc gagttttgga ggatgaagag gggtcgaagg 601 acattgaatt gtcagatgac ccTTATGACT GCATACGACT AAGTGTGGAG AATGTCCCCT 661 GCATTGTCAC TCTGTGCAAG ATTGGCTATC GGCATGTGGT GGATGCTACT CTTCAGGAGG 721 AGGCCTGCTC GCTGGCCAGC TTGCTGGTGT CGGTGACCAG CAAGGGAGTT GTGACGTGCA 781 TGAGGAAAGT GGGGAAGGGC AGCCTGGACC CAGAGAGCAT CTTCGAGATG ATGGAGACTG 841 GCAAGCGTGT GGGCAAGGTA CTGCATGCCT CCTTGCAGAG TGTTCTGCAC AAGGAAGAAA 901 GCCTGGGGCC CAAGAGACAG AAAGTTGGAT TCCTGGGATG CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGACCGAAC CAGGACAAAG AAAGTCTGAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt