Construct: ORF TRCN0000471318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002562.1_s317c1
- Derived from:
- ccsbBroadEn_07223
- DNA Barcode:
- CGGCGACCCACTCTTCACCGCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC4 (8347)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 99.7% | 99.2% | 13G>A |
| 2 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 92.8% | 100% | (many diffs) |
| 3 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 92.8% | 100% | (many diffs) |
| 4 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 92.8% | 99.2% | (many diffs) |
| 5 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 92.8% | 97.6% | (many diffs) |
| 6 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.7% | 99.2% | (many diffs) |
| 7 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 91% | 99.2% | (many diffs) |
| 8 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 90.4% | 98.4% | (many diffs) |
| 9 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 90.4% | 98.4% | (many diffs) |
| 10 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 90.4% | 97.6% | (many diffs) |
| 11 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 90.2% | 99.2% | (many diffs) |
| 12 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 90.2% | 98.4% | (many diffs) |
| 13 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 90.2% | 97.6% | (many diffs) |
| 14 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 89.9% | 99.2% | (many diffs) |
| 15 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 89.9% | 98.4% | (many diffs) |
| 16 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 89.9% | 96.8% | (many diffs) |
| 17 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 89.9% | 96.8% | (many diffs) |
| 18 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 89.4% | 97.6% | (many diffs) |
| 19 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 88.3% | 96.8% | (many diffs) |
| 20 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 86.5% | 97.6% | (many diffs) |
| 21 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 86.5% | 95.2% | (many diffs) |
| 22 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 84% | 91.7% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 78% | 84% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 67% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 57.4% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 20.9% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 18.1% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 16.9% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 15.6% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.1% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 13.6% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.2% | (many diffs) | |
| 33 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 92.5% | 100% | (many diffs) |
| 34 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 92.5% | 99.2% | (many diffs) |
| 35 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92.5% | 99.2% | (many diffs) |
| 36 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 92.5% | 99.2% | (many diffs) |
| 37 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92.5% | 99.2% | (many diffs) |
| 38 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 92.5% | 99.2% | (many diffs) |
| 39 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92.3% | 98.4% | (many diffs) |
| 40 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 92.3% | 98.4% | (many diffs) |
| 41 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 92% | 98.4% | (many diffs) |
| 42 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 92% | 98.4% | (many diffs) |
| 43 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 92% | 98.4% | (many diffs) |
| 44 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 91.7% | 99.2% | (many diffs) |
| 45 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91.5% | 98.4% | (many diffs) |
| 46 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 91.5% | 98.4% | (many diffs) |
| 47 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 91.2% | 97.6% | (many diffs) |
| 48 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.2% | 97.6% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 91.2% | 97.6% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.9% | 96.8% | (many diffs) |
| 51 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 88% | 97.6% | (many diffs) |
| 52 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 86.7% | 95.2% | (many diffs) |
| 53 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 86.7% | 93.6% | (many diffs) |
| 54 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86.3% | 91.7% | (many diffs) |
| 55 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86.3% | 91.7% | (many diffs) |
| 56 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 83% | 89.1% | (many diffs) |
| 57 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 81.7% | 86.6% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tGAGCCAACC AAGTCTGCTC CCGCCCCGAA GAAGGGCTCC AAGAAGGCAG 121 TGACCAAAGC GCAGAAGAAA GATGGCAAGA AGCGCAAGCG CAGCCGCAAG GAGAGTTACT 181 CTGTGTACGT GTACAAGGTG CTGAAACAGG TCCATCCCGA CACTGGCATC TCTTCCAAGG 241 CCATGGGCAT CATGAATTCT TTCGTTAACG ACATATTTGA GCGCATCGCG GGCGAGGCTT 301 CCCGCCTGGC GCATTACAAC AAGCGCTCGA CCATCACCTC CAGGGAGATC CAGACGGCCG 361 TGCGCCTGCT GCTTCCCGGA GAGCTGGCCA AGCACGCCGT GTCGGAGGGC ACCAAGGCCG 421 TCACCAAGTA CACCAGCTCC AAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GGCGACCCAC 601 TCTTCACCGC CCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att