Construct: ORF TRCN0000471327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005898.1_s317c1
- Derived from:
- ccsbBroadEn_07456
- DNA Barcode:
- GGAACCAGACAGATTGGCCTCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICAL2 (9645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282663.2 | 99.9% | 99.9% | 409C>A;414T>G |
| 2 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346292.2 | 99.9% | 99.9% | 409C>A;414T>G |
| 3 | human | 9645 | MICAL2 | microtubule associated mono... | NM_014632.4 | 99.9% | 99.9% | 409C>A;414T>G |
| 4 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282664.1 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 5 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346293.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 6 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346294.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 7 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346295.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 8 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346296.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 9 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346297.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 10 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346298.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 11 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001346299.2 | 98% | 98% | 409C>A;414T>G;2782_2783ins63 |
| 12 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144415.2 | 85.7% | (many diffs) | |
| 13 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144420.2 | 84.2% | (many diffs) | |
| 14 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144418.2 | 84.1% | (many diffs) | |
| 15 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144419.2 | 84.1% | (many diffs) | |
| 16 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282665.1 | 83% | 83% | 409C>A;414T>G;2212_2213ins570 |
| 17 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144416.2 | 82.6% | (many diffs) | |
| 18 | human | 9645 | MICAL2 | microtubule associated mono... | NR_144417.2 | 82.5% | (many diffs) | |
| 19 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282666.1 | 73.7% | 73.7% | (many diffs) |
| 20 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282667.1 | 72% | 71.9% | (many diffs) |
| 21 | human | 9645 | MICAL2 | microtubule associated mono... | NM_001282668.1 | 17.5% | 17.3% | (many diffs) |
| 22 | mouse | 320878 | Mical2 | microtubule associated mono... | NM_001193305.1 | 84.6% | 86.2% | (many diffs) |
| 23 | mouse | 320878 | Mical2 | microtubule associated mono... | XM_006507954.1 | 84.6% | 86.2% | (many diffs) |
| 24 | mouse | 320878 | Mical2 | microtubule associated mono... | NM_177282.5 | 62.8% | 64.3% | (many diffs) |
| 25 | mouse | 320878 | Mical2 | microtubule associated mono... | XR_001785575.1 | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3438
- ORF length:
- 3372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaaaacgag gatgagaagc aggcccaggc ggggcaggtt tttgagaact 121 ttgtccaggc atccacgtgc aaaggtaccc tccaggcctt caacattctc acacgacacc 181 tggacctaga ccctctggac cacagaaact tttattccaa gctcaagtcc aaggtgacca 241 cctggaaagc caaagccctg tggtacaaat tggataagcg tggttcccac aaagagtata 301 agcgagggaa gtcgtgcacg aacaccaagt gtctcatagt tgggggagga ccctgtggct 361 tgcgcactgc cattgaactt gcctacctgg gagccaaagt ggtcgtggtg gagaagaggg 421 actccttctc ccggaacaac gtgctacacc tctggccttt caccatccat gacattcggg 481 gcctgggagc caagaagttc tatgggaagt tctgtgctgg ctccatcgac catatcagta 541 ttcgccaact acagctcatc ctattcaagg tggccctgat gctgggagtt gaaatccatg 601 tgaatgtgga gttcgtgaag gttctagagc ctcctgaaga tcaagaaaat caaaaaattg 661 gctggcgggc agaatttctc cctacagacc attctctgtc ggagtttgag tttgacgtca 721 tcattggtgc cgatggccgc aggaacaccc tggaagggtt cagaagaaaa gaattccgtg 781 ggaagctggc gattgccatc accgccaact tcataaacag aaacagcaca gcggaagcca 841 aggtggaaga gattagtggt gtggctttca tcttcaatca gaaatttttt caggacctta 901 aagaagaaac aggcatagat cttgagaaca ttgtttacta caaggactgc acccactatt 961 ttgtaatgac agccaagaag cagagcctgc tcgacaaagg tgtcatcatt aacgactaca 1021 tcgacacaga gatgctgctg tgtgcggaga acgtgaacca agacaacctg ctatcctatg 1081 cccgggaagc tgcagacttt gccaccaact accagctgcc atccttagac tttgccatga 1141 accactatgg gcagcctgat gtggccatgt ttgactttac ctgcatgtat gcctcagaga 1201 acgcggccct ggtgcgggag cggcaggcgc accagctgct cgtggccctt gtgggtgaca 1261 gcttgcttga gccattttgg cccatgggta caggctgtgc ccgtggcttc ctggcagcct 1321 ttgacacggc atggatggtg aagagctgga accagggcac ccctcccctg gagctgctgg 1381 ctgaaaggga aagtctctac cggctgttac ctcagacaac cccggagaac atcaacaaga 1441 actttgagca gtacacgttg gacccaggga cacggtaccc aaacctcaac tcacactgtg 1501 tcaggcccca tcaggtgaag catttgtata tcactaagga gctggagcac taccctctcg 1561 agagactggg ctcggtgagg agatctgtca acctctccag gaaggagtca gatatccggc 1621 ccagcaagct cctgacctgg tgccagcagc agacagaggg ctaccagcat gtcaacgtca 1681 ccgacctgac cacatcctgg cgcagtgggt tggccctgtg tgccatcatc caccgcttcc 1741 ggcctgagct catcaacttt gactctttga atgaagatga tgctgtggag aacaaccagc 1801 tcgcatttga tgtggccgag cgagagtttg ggatccctcc agtgaccacg ggcaaagaga 1861 tggcatctgc ccaggagcct gacaagctca gcatggtcat gtacctctcc aagttctacg 1921 agctcttccg gggcacccca ctgaggcccg tggattcttg gcgcaaaaac tatggagaaa 1981 atgctgacct cagcttggcc aaatcatcca tttctaataa ctatctcaac ctcacatttc 2041 caaggaagag gactccacgg gtggatggtc aaaccggaga gaatgacatg aacaaacgga 2101 gacggaaggg cttcaccaac ctggacgagc cttcaaactt ttccagccgt agcttgggct 2161 ccaatcaaga gtgtgggagc agtaaggaag gtggaaatca gaacaaagtc aagtccatgg 2221 cgaatcagct gctggccaag tttgaggaga gcactcggaa cccctcactc atgaagcagg 2281 aacgccgtgt ctcagggata ggtaagccgg tcctgtgctc ttcctccggc cctcctgttc 2341 actcttgctg ccccaagccg gaggaggcca cacccagccc atcacctcct ctgaaaaggc 2401 agttcccctc tgtggtcgtg acggggcacg tgctcagaga gctcaagcaa gtgtctgctg 2461 gcagtgagtg cctgagcaga ccttggagag ccagagccaa gtctgaccta cagctgggtg 2521 ggacagaaaa tttcgctacc ctgccttcta cccgcccgag ggcgcaggct ctttccgggg 2581 tgctgtggcg gctgcagcaa gtggaggaaa agattctcca gaagagggct cagaacttgg 2641 ccaacaggga atttcacaca aagaacatta aggagaaggc ggctcacctt gcctccatgt 2701 ttggacacgg ggatttcccg cagaataaac tactctctaa aggcctgtct catactcatc 2761 ctccatctcc tccctctcgc cttccgtctc ctgatccagc tgcttcttcc tctccatcaa 2821 ctgttgactc tgcttctcct gccagaaagg aaaagaagtc accttcaggg ttccattttc 2881 atcccagcca tttgagaaca gtgcatcctc agctgacggt agggaaagtg tccagcggaa 2941 taggggctgc agctgaagtc ctggtcaatc tgtacatgaa tgatcacaga cctaaggccc 3001 aggccacctc tccagacctg gaatctatgc gaaagtcatt tccccttaAC CTGGGAGGCA 3061 GCGACACGTG TTACTTCTGT AAGAAACGTG TGTACGTGAT GGAACGGCTG AGCGCCGAGG 3121 GCCACTTCTT CCACCGGGAG TGTTTCCGCT GCAGCATCTG TGCCACCACC TTGCGCCTGG 3181 CCGCCTACAC CTTTGACTGC GATGAAGGCA AATTTTACTG CAAGCCTCAC TTCATTCACT 3241 GTAAAACCAA TAGCAAACAA CGGAAGAGAC GGGCAGAGTT GAAGCAACAA AGAGAGGAGG 3301 AGGCAACATG GCAAGAGCAG GAAGCCCCTC GGAGAGACAC TCCCACCGAA AGTTCTTGCG 3361 CAGTGGCCGC CATTGGCACC CTGGAAGGCA GCCCCCCAGT TCATTTCAGC CTTCCAGTGC 3421 TACACCCACT TCTTGGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGAACCA GACAGATTGG 3601 CCTCCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt