Construct: ORF TRCN0000471331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003733.1_s317c1
- Derived from:
- ccsbBroadEn_13523
- DNA Barcode:
- AGCATCAGCGTCTGGCTGGGGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN1 (284312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284312 | ZSCAN1 | zinc finger and SCAN domain... | XM_017026610.1 | 62.1% | 50.9% | (many diffs) |
| 2 | human | 284312 | ZSCAN1 | zinc finger and SCAN domain... | XM_017026609.1 | 53.7% | 44.2% | (many diffs) |
| 3 | human | 284312 | ZSCAN1 | zinc finger and SCAN domain... | NM_182572.4 | 39.6% | 32.9% | (many diffs) |
| 4 | human | 284312 | ZSCAN1 | zinc finger and SCAN domain... | XM_006723149.2 | 36.1% | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tccacggccc aaagcccctg cctcccccag acgcccccag accccaaccc 121 cgagtgagca ggacgcagac cctgggccag caagccccag ggacaccgaa gcccagcgtc 181 TGCGCTTCCG GCAGTTCCAG TACCACGTGG CGAGCGGGCC GCACCTGGCG CTGGGCCAGC 241 TCTGGACGCT GTGCCGCCAG TGGCTGAGGC CAGAGGCGCG CTCCAAGGAG CAGATGCTGG 301 AGCTGCTGGT GCTGGAGCAG TTCCTGGGCG CGCTGCCCAG CAAGATGCGG ACCTGGGTGC 361 AGTCACAGGG CCCCCGAAGC TGCAGGGAGG CCGCCAGCCT GGTGGAGGAC CTCACACAGA 421 TGTGCCAGCA GGAAGGTGAG AGGCGCAGGC TTCGTGCCCC GGGCCGGGCC AGGGGGCCTG 481 ACGTCTCCGA GGACCGAACT GCCCTCCTTC CCGCCCACAT CGCTCCCACC CCTGCCCGGC 541 CCCTCCCGAC CAGCAAACCT CTCCTGCCCC GCGGCTGTGT TTTTAATTAT TTTTACATAT 601 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAAGCAT CAGCGTCTGG CTGGGGGAAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt