Construct: ORF TRCN0000471352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012418.1_s317c1
- Derived from:
- ccsbBroadEn_07053
- DNA Barcode:
- GCCTAATAACGGGATTTTCCATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TFPI (7035)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_001032281.3 | 99.8% | 100% | 24A>C |
| 2 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_001318941.2 | 99.8% | 100% | 24A>C |
| 3 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_024453059.1 | 99.8% | 100% | 24A>C |
| 4 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_024453060.1 | 99.8% | 100% | 24A>C |
| 5 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_024453061.1 | 99.8% | 100% | 24A>C |
| 6 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_024453062.1 | 99.8% | 100% | 24A>C |
| 7 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_001329239.2 | 76.5% | 63.3% | (many diffs) |
| 8 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_001329240.2 | 76.5% | 63.3% | (many diffs) |
| 9 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_001329241.2 | 76.5% | 63.3% | (many diffs) |
| 10 | human | 7035 | TFPI | tissue factor pathway inhib... | NM_006287.6 | 76.5% | 63.3% | (many diffs) |
| 11 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_006712720.4 | 76.5% | 63.3% | (many diffs) |
| 12 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_011511709.3 | 76.5% | 63.3% | (many diffs) |
| 13 | human | 7035 | TFPI | tissue factor pathway inhib... | XM_024453058.1 | 76.5% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ttacacaatg aagaaagtcc atgcactttg ggcttctgta tgcctgctgc 121 ttaatcttgc ccctgcccct cttaatgctg attctgagga agatgaagaa cacacaatta 181 tcacagatac ggagttgcca ccactgaaac ttatgcattc attttgtgca ttcaaggcgg 241 atgatggccc atgtaaagca atcatgaaaa gatttttctt caatattttc actcgacagt 301 gcgaagaatt tatatatgGG GGATGTGAAG GAAATCAGAA TCGATTTGAA AGTCTGGAAG 361 AGTGCAAAAA AATGTGTACA AGAGATAATG CAAACAGGAT TATAAAGACA ACATTGCAAC 421 AAGAAAAGCC AGATTTCTGC TTTTTGGAAG AAGATCCTGG AATATGTCGA GGTTATATTA 481 CCAGGTATTT TTATAACAAT CAGACAAAAC AGTGTGAACG TTTCAAGTAT GGTGGATGCC 541 TGGGCAATAT GAACAATTTT GAGACACTGG AAGAATGCAA GAACATTTGT GAAGATGGTC 601 CGAATGGTTT CCAGGTGGAT AATTATGGAA CCCAGCTCAA TGCTGTGAAT AACTCCCTGA 661 CTCCGCAATC AACCAAGGTT CCCAGCCTTT TTGTTACAAA AGAAGGAACA AATGATGGTT 721 GGAAGAATGC GGCTCATATT TACCAAGTCT TTCTGAACGC CTTCTGCATT CATGCATCCA 781 TGTTCTTTCT AGGATTGGAT AGCATTTCAT GCCTATGTTA CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAGCCTAA TAACGGGATT TTCCATGGAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt