Construct: ORF TRCN0000471389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002058.1_s317c1
- Derived from:
- ccsbBroadEn_12557
- DNA Barcode:
- AGCTGGATCATAGTTCCGTCGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSD1 (79187)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79187 | FSD1 | fibronectin type III and SP... | NM_024333.3 | 62.2% | 62.2% | 1_561del |
| 2 | human | 79187 | FSD1 | fibronectin type III and SP... | NM_001330429.1 | 50.4% | 45.1% | 1_561del;1198_1199ins91;1311_1312ins86 |
| 3 | mouse | 240121 | Fsd1 | fibronectin type 3 and SPRY... | NM_183178.2 | 54.9% | 58.4% | (many diffs) |
| 4 | mouse | 240121 | Fsd1 | fibronectin type 3 and SPRY... | XR_385326.1 | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggatgaggac agcaagattg accactacgt gctggagtac cggcggacca 121 acttcgaggg cccgccccgc ctcaaggagg accagccctg gatggtcatc gagggcatcc 181 ggcagacaga gtacaccctg acaggtctca agtttgacat gaaatacatg aacttccgtg 241 tgaaggcctg taacaaggca gttgcaggag agttctctga gccggtgact ctggagacac 301 cagcgttcat gttccgcctg gatgcgtcca catcccacca gaacctgcgg gtggatgatc 361 tctccgtgga gtgggacgct atgggcggga aggtgcagga tatcaaggct cgcgagaaag 421 atggcaaggg gcggacggcg tctcccatca actccccagc cagaggtact ccatctccca 481 agaggatgcc ctcaggtcgt gggggacggg accgcttcac cgctgagtcc TACACAGTTC 541 TGGGGGACAC GCTGATCGAC GGCGGGGAGC ATTACTGGGA GGTGCGCTAC GAGCCGGACA 601 GCAAGGCGTT CGGCGTGGGC GTGGCCTACC GCAGCCTGGG CCGCTTCGAG CAACTGGGCA 661 AGACGGCCGC CTCCTGGTGC CTGCACGTCA ACAATTGGCT GCAGGTCAGC TTCACGGCCA 721 AGCACGCCAA CAAGGTCAAG GTGCTGGACG CCCCCGTGCC CGACTGCCTG GGTGTGCACT 781 GTGACTTCCA CCAAGGCCTC CTGTCCTTCT ACAATGCCCG CACCAAACAA GTGCTGCACA 841 CTTTCAAGAC CAGGTTCACA CAGCCGCTGC TGCCTGCTTT CACGGTATGG TGTGGCAGCT 901 TCCAGGTGAC GACAGGCCTG CAGGTCCCCA GTGCTGTGCG CTGCCTGCAA AAGCGAGGCA 961 GTGCTACCAG CAGCTCCAAC ACCAGCCTCA CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1141 CTGGATCATA GTTCCGTCGT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt