Construct: ORF TRCN0000471395

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011840.4_s317c1
Derived from:
ccsbBroadEn_14701
DNA Barcode:
GCTCCGGCGTTTTTCGCGCTAATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MPP2 (4355)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471395
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4355 MPP2 membrane palmitoylated prot... NM_001278381.1 99.8% 100% 381A>G;525A>G
2 human 4355 MPP2 membrane palmitoylated prot... NM_005374.5 99.8% 100% 381A>G;525A>G
3 human 4355 MPP2 membrane palmitoylated prot... XM_024450761.1 99.8% 100% 381A>G;525A>G
4 human 4355 MPP2 membrane palmitoylated prot... XM_024450762.1 99.8% 100% 381A>G;525A>G
5 human 4355 MPP2 membrane palmitoylated prot... XM_024450763.1 99.8% 100% 381A>G;525A>G
6 human 4355 MPP2 membrane palmitoylated prot... NM_001278371.1 97.8% 98% 0_1ins33;348A>G;492A>G
7 human 4355 MPP2 membrane palmitoylated prot... NM_001278373.2 97.8% 98% 0_1ins33;348A>G;492A>G
8 human 4355 MPP2 membrane palmitoylated prot... NM_001278375.1 97.8% 98% 0_1ins33;348A>G;492A>G
9 human 4355 MPP2 membrane palmitoylated prot... NM_001278372.2 95.7% 95.8% 150_221del;453A>G;597A>G
10 human 4355 MPP2 membrane palmitoylated prot... NM_001278376.3 95.5% 95.6% (many diffs)
11 human 4355 MPP2 membrane palmitoylated prot... NM_001278370.1 92.3% 92.4% 1_135del;516A>G;660A>G
12 human 4355 MPP2 membrane palmitoylated prot... XM_024450760.1 90.9% 91% 1_162del;543A>G;687A>G
13 human 4355 MPP2 membrane palmitoylated prot... XM_011524827.2 87.5% 87.6% (many diffs)
14 human 4355 MPP2 membrane palmitoylated prot... NM_001278374.2 74.7% 74.8% 0_1ins417;108A>G
15 human 4355 MPP2 membrane palmitoylated prot... XR_001752510.2 38.7% (many diffs)
16 human 4355 MPP2 membrane palmitoylated prot... XR_002958008.1 37.8% (many diffs)
17 human 4355 MPP2 membrane palmitoylated prot... XR_001752511.2 37.4% (many diffs)
18 mouse 50997 Mpp2 membrane protein, palmitoyl... NM_016695.3 89.7% 97.6% (many diffs)
19 mouse 50997 Mpp2 membrane protein, palmitoyl... XM_006533684.3 89.7% 97.6% (many diffs)
20 mouse 50997 Mpp2 membrane protein, palmitoyl... XM_006533685.3 89.7% 97.6% (many diffs)
21 mouse 50997 Mpp2 membrane protein, palmitoyl... XM_006533683.3 86.4% 93.4% (many diffs)
22 mouse 50997 Mpp2 membrane protein, palmitoyl... XM_006533687.1 67.4% 73.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1722
ORF length:
1656
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ggttgccgcc accaactctg aaactgccat gcagcaagtc ctggacaact
121tgggatccct ccccagtgcc acgggggctg cagagctgga cctgatcttc cttcgaggca
181ttatggaaag tcccatagta agatccctgg ccaaggccca tgagaggctg gaggagacga
241agctggaggc cgtgagagac aacaacctgg agctggtgca ggagatcctg cgggacctgg
301cgcagctggc tgagcagagc agcacagccg ccgagctggc ccacatcctc caggagcccc
361acttccagtc cctcctggag acgcacgact ctgtggcctc aaagacctat gagacaccac
421cccccagccc tggcctggac cctacgttca gcaaccagcc tgtacctccc gatgctgtgc
481gcatggtggg catccgcaag acagccggag aacatctggg tgtaacgttc cgcgtggagg
541gcggcgagct ggtgatcgcg cgcattctgc atgggggcat ggtggctcag caaggcctgc
601tgcatgtggg tgacatcatc aaggaggtga acgggcagcc agtgggcagt gacccccgcg
661cactgcagga gctcctgcgc aatgccagtg gcagtgtcat cctcaagatc ctgcccagct
721accaggagcc ccatctgccc cgccaggtat ttgtgaaatg tcactttgac tatgacccgg
781cccgagacag cctcatcccc tgcaaggaag caggcctgcg cttcaacgcc ggggacttgc
841tccagatcgt aaaccaggat gatgccaact ggtggcaggc atgccatgtc gaagggggca
901gtgctgggct cattcccagc cagctgctgg aggagaagcg gaaagcattt gtcaagaggg
961acctggagct gacaccaaac tcagggaccc tatgcggcag cctttcagga aagaaaaaga
1021agcgaatgat gtatttgacc accaagaatg cagagtttga ccgtcatgag ctgctcattt
1081atgaggaggt ggcccgcatg cccccgttcc gccggaaaac cctggtactg attggggctc
1141agggcgtggg acggcgcagc ctgaagaaca agctcatcat gtgggatcca gatcgctatg
1201gcaccacggt gccctacacc tcccggcggc cgaaagactc agagcgggaa ggtcagggtt
1261acagctttgt gtcCCGTGGG GAGATGGAGG CTGACGTCCG TGCTGGGCGC TACCTGGAGC
1321ATGGCGAATA CGAGGGCAAC CTGTATGGCA CACGTATTGA CTCCATCCGG GGCGTGGTCG
1381CTGCTGGGAA GGTGTGCGTG CTGGATGTCA ACCCCCAGGC GGTGAAGGTG CTACGAACGG
1441CCGAGTTTGT CCCTTACGTG GTGTTCATCG AGGCCCCAGA CTTCGAGACC CTGCGGGCCA
1501TGAACAGGGC TGCGCTGGAG AGTGGAATAT CCACCAAGCA GCTCACGGAG GCGGACCTGA
1561GACGGACAGT GGAGGAGAGC AGCCGCATCC AGCGGGGCTA CGGGCACTAC TTTGACCTCT
1621GCCTGGTCAA TAGCAACCTG GAGAGGACCT TCCGCGAGCT CCAGACAGCC ATGGAGAAGC
1681TACGGACAGA GCCCCAGTGG GTGCCTGTCA GCTGGGTGTA CTGCCCAACT TTCTTGTACA
1741AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1801AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1861AGGACGAGCT CCGGCGTTTT TCGCGCTAAT TACGCGTTAA GTCgacaatc aacctctgga
1921ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)