Construct: ORF TRCN0000471409
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016301.1_s317c1
- Derived from:
- ccsbBroadEn_10924
- DNA Barcode:
- ATGATTAATCTAATGAATATATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISG20 (3669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471409
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303237.2 | 52% | 45.2% | (many diffs) |
| 2 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_017022148.1 | 52% | 45.2% | (many diffs) |
| 3 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303233.2 | 44.9% | 42.5% | (many diffs) |
| 4 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_001303234.2 | 44.9% | 42.5% | (many diffs) |
| 5 | human | 3669 | ISG20 | interferon stimulated exonu... | NM_002201.6 | 44.9% | 42.5% | (many diffs) |
| 6 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_005254899.2 | 44.9% | 42.5% | (many diffs) |
| 7 | human | 3669 | ISG20 | interferon stimulated exonu... | XM_006720488.3 | 44.9% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 327
- ORF length:
- 261
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggagccgt gaggtggtgg ccatggactg cgagatggtg gggctggggc 121 cCCACCGGGA GAGTGGCCTG GCTCGTTGCA GCCTCGTGAA CGTCCACGGT GCTGTGCTGT 181 ACGACAAGTT CATCCGGCCT GAGGGAGAGA TCACCGATTA CAGAACCCGG GTCAGCGGGG 241 TCACCCCTCA GCACATGGTG GGGGCCACAC CATTTGCCGT GGCCAGGCTA GAGGTCCCCT 301 TCCCATCCTC TCCAACGGCA GCTGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 361 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 421 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGATTAA 481 TCTAATGAAT ATATATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 541 aagatt