Construct: ORF TRCN0000471437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018366.1_s317c1
- Derived from:
- ccsbBroadEn_09142
- DNA Barcode:
- TGCCTCGGTCCCGTGACACTAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCALD (83988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83988 | NCALD | neurocalcin delta | NM_001040624.1 | 99.8% | 99.4% | 496C>A |
| 2 | human | 83988 | NCALD | neurocalcin delta | NM_001040625.1 | 99.8% | 99.4% | 496C>A |
| 3 | human | 83988 | NCALD | neurocalcin delta | NM_001040626.1 | 99.8% | 99.4% | 496C>A |
| 4 | human | 83988 | NCALD | neurocalcin delta | NM_001040627.1 | 99.8% | 99.4% | 496C>A |
| 5 | human | 83988 | NCALD | neurocalcin delta | NM_001040628.1 | 99.8% | 99.4% | 496C>A |
| 6 | human | 83988 | NCALD | neurocalcin delta | NM_001040629.1 | 99.8% | 99.4% | 496C>A |
| 7 | human | 83988 | NCALD | neurocalcin delta | NM_001040630.1 | 99.8% | 99.4% | 496C>A |
| 8 | human | 83988 | NCALD | neurocalcin delta | NM_032041.3 | 99.8% | 99.4% | 496C>A |
| 9 | human | 83988 | NCALD | neurocalcin delta | XM_011517332.3 | 99.8% | 99.4% | 496C>A |
| 10 | human | 83988 | NCALD | neurocalcin delta | XM_011517333.2 | 99.8% | 99.4% | 496C>A |
| 11 | human | 83988 | NCALD | neurocalcin delta | XM_011517334.2 | 99.8% | 99.4% | 496C>A |
| 12 | human | 83988 | NCALD | neurocalcin delta | XM_011517335.2 | 99.8% | 99.4% | 496C>A |
| 13 | human | 83988 | NCALD | neurocalcin delta | XM_017013901.1 | 99.8% | 99.4% | 496C>A |
| 14 | human | 83988 | NCALD | neurocalcin delta | XM_024447296.1 | 99.8% | 99.4% | 496C>A |
| 15 | human | 83988 | NCALD | neurocalcin delta | XM_024447297.1 | 99.8% | 99.4% | 496C>A |
| 16 | human | 83988 | NCALD | neurocalcin delta | XM_024447298.1 | 99.8% | 99.4% | 496C>A |
| 17 | human | 83988 | NCALD | neurocalcin delta | XM_024447299.1 | 99.8% | 99.4% | 496C>A |
| 18 | human | 83988 | NCALD | neurocalcin delta | XM_024447300.1 | 99.8% | 99.4% | 496C>A |
| 19 | human | 83988 | NCALD | neurocalcin delta | XM_024447301.1 | 99.8% | 99.4% | 496C>A |
| 20 | human | 83988 | NCALD | neurocalcin delta | XM_024447302.1 | 99.8% | 99.4% | 496C>A |
| 21 | human | 83988 | NCALD | neurocalcin delta | XM_024447303.1 | 99.8% | 99.4% | 496C>A |
| 22 | human | 83988 | NCALD | neurocalcin delta | XM_024447304.1 | 99.8% | 99.4% | 496C>A |
| 23 | human | 3241 | HPCAL1 | hippocalcin like 1 | NM_001258357.1 | 80.5% | 90.1% | (many diffs) |
| 24 | human | 3241 | HPCAL1 | hippocalcin like 1 | NM_001258358.1 | 80.5% | 90.1% | (many diffs) |
| 25 | human | 3241 | HPCAL1 | hippocalcin like 1 | NM_001258359.1 | 80.5% | 90.1% | (many diffs) |
| 26 | human | 3241 | HPCAL1 | hippocalcin like 1 | NM_002149.4 | 80.5% | 90.1% | (many diffs) |
| 27 | human | 3241 | HPCAL1 | hippocalcin like 1 | NM_134421.2 | 80.5% | 90.1% | (many diffs) |
| 28 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_005246160.1 | 80.5% | 90.1% | (many diffs) |
| 29 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_005246161.1 | 80.5% | 90.1% | (many diffs) |
| 30 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_005246162.1 | 80.5% | 90.1% | (many diffs) |
| 31 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_005246163.1 | 80.5% | 90.1% | (many diffs) |
| 32 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_011510346.2 | 80.5% | 90.1% | (many diffs) |
| 33 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_011510347.1 | 80.5% | 90.1% | (many diffs) |
| 34 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_011510348.1 | 80.5% | 90.1% | (many diffs) |
| 35 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_017003950.1 | 80.5% | 90.1% | (many diffs) |
| 36 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_017003951.1 | 80.5% | 90.1% | (many diffs) |
| 37 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_017003952.1 | 80.5% | 90.1% | (many diffs) |
| 38 | human | 3241 | HPCAL1 | hippocalcin like 1 | XM_017003953.2 | 80.5% | 90.1% | (many diffs) |
| 39 | mouse | 52589 | Ncald | neurocalcin delta | NM_001170866.1 | 92.4% | 99.4% | (many diffs) |
| 40 | mouse | 52589 | Ncald | neurocalcin delta | NM_001170867.1 | 92.4% | 99.4% | (many diffs) |
| 41 | mouse | 52589 | Ncald | neurocalcin delta | NM_001170868.1 | 92.4% | 99.4% | (many diffs) |
| 42 | mouse | 52589 | Ncald | neurocalcin delta | NM_134094.4 | 92.4% | 99.4% | (many diffs) |
| 43 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520107.3 | 92.4% | 99.4% | (many diffs) |
| 44 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520108.1 | 92.4% | 99.4% | (many diffs) |
| 45 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520109.2 | 92.4% | 99.4% | (many diffs) |
| 46 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520111.3 | 92.4% | 99.4% | (many diffs) |
| 47 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520112.3 | 92.4% | 99.4% | (many diffs) |
| 48 | mouse | 52589 | Ncald | neurocalcin delta | XM_006520113.3 | 92.4% | 99.4% | (many diffs) |
| 49 | mouse | 52589 | Ncald | neurocalcin delta | XM_011245362.2 | 92.4% | 99.4% | (many diffs) |
| 50 | mouse | 52589 | Ncald | neurocalcin delta | XM_011245363.2 | 92.4% | 99.4% | (many diffs) |
| 51 | mouse | 52589 | Ncald | neurocalcin delta | XM_011245364.2 | 92.4% | 99.4% | (many diffs) |
| 52 | mouse | 52589 | Ncald | neurocalcin delta | XM_011245365.2 | 92.4% | 99.4% | (many diffs) |
| 53 | mouse | 52589 | Ncald | neurocalcin delta | XM_011245366.2 | 92.4% | 99.4% | (many diffs) |
| 54 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316691.1 | 92.4% | 99.4% | (many diffs) |
| 55 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316692.1 | 92.4% | 99.4% | (many diffs) |
| 56 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316693.1 | 92.4% | 99.4% | (many diffs) |
| 57 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316694.1 | 92.4% | 99.4% | (many diffs) |
| 58 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316695.1 | 92.4% | 99.4% | (many diffs) |
| 59 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316696.1 | 92.4% | 99.4% | (many diffs) |
| 60 | mouse | 52589 | Ncald | neurocalcin delta | XM_017316697.1 | 92.4% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 645
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaaacagaac agcaagctgc gcccggaggt catgcaggac ttgctggaaa 121 gcacagactt tacagagcat gagatccagg aatggtataa aggcttcttg agagactgcc 181 cCAGTGGACA TTTGTCAATG GAAGAGTTTA AGAAAATATA TGGGAACTTT TTCCCTTATG 241 GGGATGCTTC CAAATTTGCA GAGCATGTCT TCCGCACCTT CGATGCAAAT GGAGATGGGA 301 CAATAGACTT TAGAGAATTC ATCATCGCCT TGAGTGTAAC TTCGAGGGGG AAGCTGGAGC 361 AGAAGCTGAA ATGGGCCTTC AGCATGTACG ACCTGGACGG AAATGGCTAT ATCAGCAAGG 421 CAGAGATGCT AGAGATCGTG CAGGCAATCT ATAAGATGGT TTCCTCTGTA ATGAAAATGC 481 CTGAAGATGA GTCAACCCCA GAGAAAAGAA CAGAAAAGAT CTTCCGCCAG ATGGACACCA 541 ATAGAGACGG AAAACTCTCC ATGGAAGAGT TCATCCGAGG AGCCAAAAGC GACCCGTCCA 601 TTGTGCGCCT CCTGCAGTGC GACCCGAGCA GTGCCGGCCA GTTCTGCCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA TGCCTCGGTC CCGTGACACT AACAACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt