Transcript: Human XM_005246162.1

PREDICTED: Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HPCAL1 (3241)
Length:
1953
CDS:
576..1157

Additional Resources:

NCBI RefSeq record:
XM_005246162.1
NBCI Gene record:
HPCAL1 (3241)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005246162.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370581 GCCGCTTGCACGTATAGATAC pLKO_005 1621 3UTR 100% 10.800 15.120 N HPCAL1 n/a
2 TRCN0000056366 GAACACGGAGTTCACCGACCA pLKO.1 629 CDS 100% 0.720 1.008 N HPCAL1 n/a
3 TRCN0000363583 GAACACGGAGTTCACCGACCA pLKO_005 629 CDS 100% 0.720 1.008 N HPCAL1 n/a
4 TRCN0000312681 CAGGCTTGTCGTGCCGTTTAA pLKO_005 1190 3UTR 100% 13.200 9.240 N HPCAL1 n/a
5 TRCN0000312680 GGCAGATGGACACCAACAATG pLKO_005 1036 CDS 100% 10.800 7.560 N HPCAL1 n/a
6 TRCN0000377587 GACCGTGGACGAGTTCAAGAA pLKO_005 704 CDS 100% 4.950 3.465 N HPCAL1 n/a
7 TRCN0000056363 GACACCAACAATGACGGCAAA pLKO.1 1044 CDS 100% 4.050 2.835 N HPCAL1 n/a
8 TRCN0000056364 CAAACTGTCCTTGGAAGAATT pLKO.1 1061 CDS 100% 0.000 0.000 N HPCAL1 n/a
9 TRCN0000327891 CAAACTGTCCTTGGAAGAATT pLKO_005 1061 CDS 100% 0.000 0.000 N HPCAL1 n/a
10 TRCN0000056365 CCCGGAGAAGCGCACAGACAA pLKO.1 1007 CDS 100% 0.000 0.000 N HPCAL1 n/a
11 TRCN0000056367 CGACGCTTCCAAGTTCGCCGA pLKO.1 752 CDS 100% 0.000 0.000 N HPCAL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005246162.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04313 pDONR223 100% 80.5% 90.6% None (many diffs) n/a
2 ccsbBroad304_04313 pLX_304 0% 80.5% 90.6% V5 (many diffs) n/a
3 TRCN0000473557 GCAATCGGCAAAAGCGCAATCTTG pLX_317 62.9% 80.5% 90.6% V5 (many diffs) n/a
4 ccsbBroadEn_09142 pDONR223 100% 80.5% 90.1% None (many diffs) n/a
5 ccsbBroad304_09142 pLX_304 0% 80.5% 90.1% V5 (many diffs) n/a
6 TRCN0000471437 TGCCTCGGTCCCGTGACACTAACA pLX_317 79.1% 80.5% 90.1% V5 (many diffs) n/a
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