Transcript: Human XM_024447297.1

PREDICTED: Homo sapiens neurocalcin delta (NCALD), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NCALD (83988)
Length:
3607
CDS:
262..843

Additional Resources:

NCBI RefSeq record:
XM_024447297.1
NBCI Gene record:
NCALD (83988)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024447297.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156703 CCTGGACGGAAATGGCTATAT pLKO.1 588 CDS 100% 13.200 18.480 N NCALD n/a
2 TRCN0000152815 GCCCAAATTCTCAAGGTTGTT pLKO.1 2409 3UTR 100% 4.950 6.930 N NCALD n/a
3 TRCN0000151437 GCATGTTTAGTGAGCACTAAT pLKO.1 1639 3UTR 100% 1.320 1.848 N NCALD n/a
4 TRCN0000153750 CCAGGAATGGTATAAAGGCTT pLKO.1 342 CDS 100% 2.640 2.112 N NCALD n/a
5 TRCN0000150364 CAAATTTGCAGAGCATGTCTT pLKO.1 447 CDS 100% 4.950 3.465 N NCALD n/a
6 TRCN0000153505 CATGAGATCCAGGAATGGTAT pLKO.1 334 CDS 100% 4.950 3.465 N NCALD n/a
7 TRCN0000153990 CCACAGAAAGAGAGACATCAT pLKO.1 2513 3UTR 100% 4.950 3.465 N NCALD n/a
8 TRCN0000152345 CCTGTTAAAGAATCAGAGGTT pLKO.1 2132 3UTR 100% 2.640 1.848 N NCALD n/a
9 TRCN0000152582 GCAGGCAATCTATAAGATGGT pLKO.1 636 CDS 100% 2.640 1.848 N NCALD n/a
10 TRCN0000104699 GCTTCCAAATTTGCAGAGCAT pLKO.1 442 CDS 100% 2.640 1.848 N Ncald n/a
11 TRCN0000327293 GCTTCCAAATTTGCAGAGCAT pLKO_005 442 CDS 100% 2.640 1.848 N Ncald n/a
12 TRCN0000150952 GAGATGGGACAATAGACTTTA pLKO.1 488 CDS 100% 13.200 7.920 N NCALD n/a
13 TRCN0000152953 GCAAATGGAGATGGGACAATA pLKO.1 481 CDS 100% 13.200 7.920 N NCALD n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024447297.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04313 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04313 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473557 GCAATCGGCAAAAGCGCAATCTTG pLX_317 62.9% 100% 100% V5 n/a
4 ccsbBroadEn_09142 pDONR223 100% 99.8% 99.4% None 496C>A n/a
5 ccsbBroad304_09142 pLX_304 0% 99.8% 99.4% V5 496C>A n/a
6 TRCN0000471437 TGCCTCGGTCCCGTGACACTAACA pLX_317 79.1% 99.8% 99.4% V5 496C>A n/a
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