Construct: ORF TRCN0000471474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004471.1_s317c1
- Derived from:
- ccsbBroadEn_16012
- DNA Barcode:
- CTGAAGCACGCGATAGCGACTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSC22D4 (81628)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81628 | TSC22D4 | TSC22 domain family member 4 | NM_001303043.2 | 99.9% | 100% | 1051T>C |
| 2 | human | 81628 | TSC22D4 | TSC22 domain family member 4 | NM_030935.5 | 99.9% | 100% | 1051T>C |
| 3 | human | 81628 | TSC22D4 | TSC22 domain family member 4 | NR_130118.2 | 52.3% | (many diffs) | |
| 4 | human | 81628 | TSC22D4 | TSC22 domain family member 4 | NR_130119.2 | 52.2% | (many diffs) | |
| 5 | mouse | 78829 | Tsc22d4 | TSC22 domain family, member 4 | NM_023910.6 | 84% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgggggcaag aagaagagta gtttccaaat caccagcgtc accacggact 121 atgagggccc tgggagccca ggggcttcgg atccccctac cccacagccc ccaaccgggc 181 ccccgccccg cctgcccaat ggggagccca gccccgatcc ggggggcaag ggcacccccc 241 ggaatggctc cccaccacct ggggcccctt cctcccgttt ccgggtggtg aagctgcccc 301 acggcctggg agagccttat cgccgcggtc gctggacgtg tgtggatgtt tatgagcgag 361 acctggagcc ccacagcttc ggcggactcc tggagggaat tcgaggggcc tcagggggcg 421 ccgggggcag atctttggat tccaggttgg agctggccag cctcggcctg ggcgccccca 481 ccccaccgtc aggcctgtct cagggcccca cctcctggct ccgtccaccc cccacctctc 541 ctggacctca ggcccgctcc ttcactgggg gactgggcca gctggtggtg cccagcaaag 601 ccaaggcaga gaaaccccca ctgtcggcct cctcacccca gcagcgcccc ccagagcctg 661 agaccggtga gagtgcgggc acatcccggg ctgccacgcc cctgccctct ctgagggtgg 721 aagcggaggc tgggggctca ggggccagga cccctccact gtcccggAGG AAAGCTGTAG 781 ACATGCGGCT GCGGATGGAG TTGGGTGCTC CAGAAGAGAT GGGGCAGGTG CCCCCACTTG 841 ACTCTCGCCC CAGCTCCCCA GCCCTCTACT TCACCCACGA TGCCAGCCTG GTTCACAAAT 901 CTCCAGACCC CTTCGGAGCA GTAGCAGCTC AGAAGTTCAG CCTGGCCCAC TCCATGTTGG 961 CCATCAGTGG TCACCTAGAC AGCGACGATG ATAGTGGCTC CGGAAGCCTG GTTGGCATTG 1021 ACAACAAAAT CGAGCAAGCC ATGGACTTGG TGAAGTCCCA CCTCATGTTT GCGGTCCGGG 1081 AGGAGGTGGA GGTGCTGAAG GAGCAGATCC GGGAACTGGC GGAGCGGAAC GCTGCGCTGG 1141 AGCAGGAGAA TGGGCTGCTG CGCGCCCTGG CCAGCCCGGA GCAGCTGGCT CAGCTGCCCT 1201 CCTCGGGGGT CCCACGGCTT GGGCCCCCTG CGCCCAATGG GCCCTCCGTC TGCCCAACTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGACTGA AGCACGCGAT AGCGACTGAG ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt