Construct: ORF TRCN0000471484
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000498.1_s317c1
- Derived from:
- ccsbBroadEn_03990
- DNA Barcode:
- ATCCAAATGCGCGCCAGTTGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS11 (64963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471484
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_022839.5 | 100% | 100% | |
| 2 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321970.2 | 99.4% | 99.4% | 64_65insGCA |
| 3 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_176805.4 | 82.9% | 82.9% | 182_183ins99 |
| 4 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321972.2 | 77.5% | 68.1% | (many diffs) |
| 5 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NM_001321974.2 | 34.6% | 31.9% | (many diffs) |
| 6 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135917.2 | 16.5% | 1_10delAATTCAAGTC;189_190insCAGCATTTACCCTCCCATTC;573_3372del | |
| 7 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135915.2 | 15.4% | (many diffs) | |
| 8 | human | 64963 | MRPS11 | mitochondrial ribosomal pro... | NR_135918.2 | 15.3% | 1_10delAATTCAAGTC;291_292ins62;531_3330del | |
| 9 | mouse | 67994 | Mrps11 | mitochondrial ribosomal pro... | XM_006541135.3 | 51.7% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggctgtgaga aacgcggggt cgcggttcct gcggtcctgg acttggcccc 121 agacagccgg cagggtcgtg gccagaacgc cggccgggac catctgcaca ggcgctcgac 181 agctcCAAGA CGCTGCGGCC AAGCAGAAAG TTGAACAGAA CGCGGCTCCC AGCCACACCA 241 AGTTCAGCAT TTACCCTCCC ATTCCAGGAG AGGAGAGCTC TCTGAGGTGG GCAGGAAAGA 301 AATTTGAGGA GATCCCAATT GCACACATTA AAGCATCCCA CAACAACACA CAGATCCAGG 361 TAGTCTCTGC TAGTAATGAG CCCCTTGCCT TTGCTTCCTG TGGCACAGAG GGATTTCGGA 421 ATGCCAAGAA GGGCACAGGC ATCGCAGCAC AGACAGCAGG CATAGCCGCA GCGGCGAGAG 481 CTAAACAAAA GGGCGTGATC CACATCCGAG TTGTGGTGAA AGGCCTGGGG CCAGGACGCT 541 TGTCTGCCAT GCACGGACTG ATCATGGGCG GCCTGGAAGT GATCTCAATC ACAGACAACA 601 CCCCAATCCC ACACAACGGC TGCCGCCCCA GGAAGGCTCG GAAGCTGTGC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGAATCCAAA TGCGCGCCAG TTGCCTAACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt