Construct: ORF TRCN0000471493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004886.1_s317c1
- Derived from:
- ccsbBroadEn_10062
- DNA Barcode:
- CACCGGATCCAACTCAATTTTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D26 (353149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353149 | TBC1D26 | TBC1 domain family member 26 | NM_178571.4 | 99.7% | 99.2% | 617C>T;700G>A |
| 2 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | NM_001039397.2 | 76.4% | 73.7% | (many diffs) |
| 3 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_011523781.2 | 70.2% | 58.3% | (many diffs) |
| 4 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_024450684.1 | 70.2% | 58.3% | (many diffs) |
| 5 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024422.1 | 63.8% | 47.1% | (many diffs) |
| 6 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_011523783.3 | 60.7% | 52.8% | (many diffs) |
| 7 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024416.2 | 60.3% | 45.8% | (many diffs) |
| 8 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024417.2 | 60.3% | 45.8% | (many diffs) |
| 9 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024418.2 | 60.3% | 45.8% | (many diffs) |
| 10 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024419.2 | 60.3% | 45.8% | (many diffs) |
| 11 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024420.1 | 60.3% | 45.8% | (many diffs) |
| 12 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024421.2 | 60.3% | 45.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 816
- ORF length:
- 750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gatggatggg gacccgtata acctgcctgc ccaggggcaa ggcaatatca 121 tcattactaa gtatgagcag ggacaccgag ctggggcagc agtggacttg gggcatgagc 181 aggttgatgt cagaaaatac accaataacc tcgggattgt gcatgagatg gagctgcccc 241 gcgtcagtgc cctggaggtg aagcaaagac gcaaggaaag taaacgtacc aacaagtggc 301 aaaagatgct tgcagactgg acaaaatata ggagcaccaa gaagctgtct caaagagtat 361 acaaagtcat tcccctGGCG GTACGGGGCC GGGCGTGGTC ACTTTTGCTA GATATTGACA 421 GAATCAAGTC CCAGAACCCA GGCAAATATA AGGTCATGAA GGAGAAGGGC AAGAGGTCCT 481 CCAGAATCAT CCACTGCATC CAGCTAGATG TCAGCCACAC CCTGCAGAAA CACATGATGT 541 TCATACAAAG ATTCGGAGTC AAGCAGCAGG AATTATGTGA CATCCTCGTG GCCTATTCTG 601 CATATAACCC TGAGGTGGGC TACCACAGGG ACCTGAGCCG CATCACTGCC ATCCTCCTCC 661 TGTGTCTGCC AGAGGAAGAT GTTTTCTGGG CGCTTACCCA GTTGCTCGCT GGTGAGAGGC 721 ACTCCCTGTG GTATTCTACA GCCCAAATAC TGCCTGGCTC GAGAAGCTCC TATCGCACCA 781 GGAGCAGGTG CTGCACAAGT CCTTCCCAAA GATCATGCCC AACTTTCTTG TACAAAGTGG 841 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 901 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 961 ACACCGGATC CAACTCAATT TTTTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1021 aatttgtgaa agatt