Construct: ORF TRCN0000471494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012074.1_s317c1
- Derived from:
- ccsbBroadEn_12531
- DNA Barcode:
- GCATGTACCGCGGGTTGGTACAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHNAK (79026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_024060.4 | 99.3% | 99.3% | 7_8insAGG |
| 2 | human | 79026 | AHNAK | AHNAK nucleoprotein | XM_017018270.1 | 2.4% | 2% | (many diffs) |
| 3 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001346445.2 | 2.3% | 2% | (many diffs) |
| 4 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001346446.2 | 2.3% | 2% | (many diffs) |
| 5 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001620.3 | 2.3% | 2% | (many diffs) |
| 6 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_001039959.2 | 89.1% | 90.6% | (many diffs) |
| 7 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_001286518.1 | 77.2% | 78% | (many diffs) |
| 8 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NR_104460.1 | 33.3% | (many diffs) | |
| 9 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877684.3 | 30.2% | (many diffs) | |
| 10 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877685.3 | 27.1% | (many diffs) | |
| 11 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877686.3 | 26.5% | (many diffs) | |
| 12 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_006527256.4 | 4% | 3.5% | (many diffs) |
| 13 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_017318272.2 | 2.9% | 2.5% | (many diffs) |
| 14 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_009643.2 | 2.3% | 2.1% | (many diffs) |
| 15 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_006527253.4 | 2.3% | 2.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgga gaaggaggag gagacaaccc gggagctgct gctgcccaac tggcagggta 121 gtggctccca cgggctgacc atcgcccaga gggacgacgg cgtctttgtg caggaggtga 181 cgcagaactc ccctgcggcc cgcactgGGG TGGTCAAGGA GGGGGACCAG ATTGTGGGTG 241 CCACCATCTA CTTTGACAAC CTGCAGTCGG GTGAGGTGAC CCAGCTGCTG AACACCATGG 301 GGCACCACAC GGTGGGCCTG AAGCTGCACC GCAAGGGGGA CCGCTCTCCC GAGCCTGGCC 361 AGACCTGGAC CCGTGAAGTC TTCAGCTCCT GCAGCTCTGA AGTGGTTCTG AACACACCAC 421 AGCCATCAGC ACTGGAATGC AAAGACCAGA ACAAACAGAA GGAAGCCAGC AGCCAAGCCG 481 GGGCAGTTTC AGTCTCCACC CCAAATGCAG GACTGTACCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 AGCATGTACC GCGGGTTGGT ACAGGACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt