Construct: ORF TRCN0000471505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009854.1_s317c1
- Derived from:
- ccsbBroadEn_04088
- DNA Barcode:
- GCTAAGCCCAAGAACTTAACACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM118B (79607)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471505
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79607 | FAM118B | family with sequence simila... | NM_024556.4 | 100% | 100% | |
| 2 | human | 79607 | FAM118B | family with sequence simila... | XM_011542977.3 | 100% | 100% | |
| 3 | human | 79607 | FAM118B | family with sequence simila... | NM_001330446.1 | 99.7% | 99.7% | 979_980insCAG |
| 4 | human | 79607 | FAM118B | family with sequence simila... | XM_017018285.2 | 99.7% | 99.7% | 979_980insCAG |
| 5 | human | 79607 | FAM118B | family with sequence simila... | XM_011542978.3 | 49.3% | 45.6% | 0_1ins511;55_100del |
| 6 | human | 79607 | FAM118B | family with sequence simila... | XM_017018286.1 | 49% | 45.3% | 0_1ins511;55_100del;514_515insCAG |
| 7 | mouse | 109229 | Fam118b | family with sequence simila... | NM_001286604.1 | 91.6% | 98% | (many diffs) |
| 8 | mouse | 109229 | Fam118b | family with sequence simila... | NM_194257.2 | 91.6% | 98% | (many diffs) |
| 9 | mouse | 109229 | Fam118b | family with sequence simila... | XM_006509937.3 | 91.6% | 98% | (many diffs) |
| 10 | mouse | 109229 | Fam118b | family with sequence simila... | XM_011242382.2 | 91.6% | 98% | (many diffs) |
| 11 | mouse | 109229 | Fam118b | family with sequence simila... | XM_006509938.2 | 91.3% | 97.7% | (many diffs) |
| 12 | mouse | 109229 | Fam118b | family with sequence simila... | NM_175411.5 | 71.2% | 76.3% | (many diffs) |
| 13 | mouse | 109229 | Fam118b | family with sequence simila... | XM_006509939.2 | 63% | 64.4% | (many diffs) |
| 14 | mouse | 109229 | Fam118b | family with sequence simila... | XM_006509940.3 | 55.6% | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttctacaggg agccaggcct ctgatataga cgagattttt ggattcttca 121 acgatggcga acctcccacc aaaaagccca ggaagctgct tccaagctta aaaactaaga 181 agcctcgaga acttgtgcta gtgattggaa caggcattag tgctgcagtt gcgccccaag 241 ttccagccct caaatcctgg aaggggttaa ttcaggcctt actggatgct gccattgatt 301 ttgatctttt agaagatgag gagagcaaaa agtttcagaa atgtctccat gaagacaaga 361 acctggtcca tgttgcccat gaccttatcc agaaactctc tcctcgtacc agtaatgttc 421 gatccacatt tttcaaggac tgtttatatg aagtatttga tgacttggag tcaaagatgg 481 aagattctgg aaaacagcta cttcagtcag ttctccacct gatggaaaat ggagccctcg 541 tattaactac aaattttgat aatctcttgg aactgtatgc agcagatcag gggaaacagc 601 ttgaatccct tgaccTTACT GATGAGAAAA AGGTCCTCGA GTGGGCTCAG GAGAAGCGTA 661 AGCTGAGCGT GTTGCATATT CACGGAGTCT ACACCAACCC TAGTGGCATT GTCCTTCATC 721 CGGCTGGATA TCAGAACGTG CTCAGGAACA CTGAAGTCAT GAGAGAAATT CAGAAACTCT 781 ACGAAAACAA GTCATTTCTT TTCCTGGGCT GTGGCTGGAC TGTGGATGAC ACCACTTTCC 841 AGGCCCTTTT CTTGGAGGCT GTCAAGCATA AATCTGACCT AGAACATTTC ATGCTGGTTC 901 GGAGAGGAGA CGTAGATGAG TTCAAAAAGC TTCGAGAAAA CATGCTGGAC AAGGGGATTA 961 AAGTCATCTC CTATGGAGAT GACTATGCCG ATCTTCCAGA ATATTTCAAG CGACTGACAT 1021 GTGAGATCTC CACAAGGGGT ACATCAGCAG GGATGGTGAG AGAAGGTCAG CTAAATGGCT 1081 CATCTGCAGC ACACAGTGAA ATAAGAGGCT GTAGTACATG CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAGCTAAG CCCAAGAACT TAACACGTAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt