Construct: ORF TRCN0000471544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001541.1_s317c1
- Derived from:
- ccsbBroadEn_15807
- DNA Barcode:
- GACAGGCTGAGTGCTACTGTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POM121L8P (29797)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25812 | POM121L1P | POM121 transmembrane nucleo... | NR_024591.1 | 7.5% | (many diffs) | |
| 2 | human | 29774 | POM121L9P | POM121 transmembrane nucleo... | NR_003714.1 | 5.6% | (many diffs) | |
| 3 | human | 102725072 | LOC102725072 | Putative uncharacterized pr... | NR_135922.1 | 5.1% | 1_3255del;3297G>A;3562_5935del | |
| 4 | human | 29797 | POM121L8P | POM121 transmembrane nucleo... | NR_024583.1 | 3.9% | (many diffs) | |
| 5 | human | 646074 | POM121L10P | POM121 transmembrane nucleo... | NR_024593.1 | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 399
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAAGAA AAGACACAGA GAAGGATGTG 121 ACATGCCTGG GCCATGGAGC ACTCTGAGAT CTCATCGCGG ACACCACTGC CCACACCTCC 181 ATCCCGTCCT GCGCAGGCCG ACACTCACTG ACGTTGAAGG CTGCCTTCAG TGCCTGGATG 241 TCTGCGGCCA CCCCAGACAT GCGGTAGATG CCCACCTCCT CCATGCCTCG GCGCTCGATC 301 TCCTCCACGC ACTGGCGCAC GATGTAGGGC ACCTTGGACC TCTCTCTCCT GCGGGAGGAG 361 GGAATGTTCT CAGTGTCCTA ACAGCCCTGC TTGGGCCATT GCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAGACAGG CTGAGTGCTA CTGTGTTAAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt