Construct: ORF TRCN0000471561

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001708.1_s317c1
Derived from:
ccsbBroadEn_08131
DNA Barcode:
TATCCAGAATTGACTTTCCCCTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SNX10 (29887)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471561
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29887 SNX10 sorting nexin 10 NM_001199835.1 99.6% 99.5% 9G>A;340G>T
2 human 29887 SNX10 sorting nexin 10 NM_001318199.2 99.6% 99.5% 9G>A;340G>T
3 human 29887 SNX10 sorting nexin 10 NM_013322.2 99.6% 99.5% 9G>A;340G>T
4 human 29887 SNX10 sorting nexin 10 XM_006715712.2 99.6% 99.5% 9G>A;340G>T
5 human 29887 SNX10 sorting nexin 10 NM_001199837.2 97.6% 97% (many diffs)
6 human 29887 SNX10 sorting nexin 10 NM_001318198.1 87.6% 85.4% (many diffs)
7 human 29887 SNX10 sorting nexin 10 NM_001362753.1 87.6% 85.4% (many diffs)
8 human 29887 SNX10 sorting nexin 10 NM_001362754.1 87.6% 85.4% (many diffs)
9 human 29887 SNX10 sorting nexin 10 XM_017012086.1 87.6% 85.4% (many diffs)
10 human 29887 SNX10 sorting nexin 10 NM_001199838.1 58% 57.7% 0_1ins252;88G>T
11 mouse 71982 Snx10 sorting nexin 10 NM_001127348.1 88.4% 95% (many diffs)
12 mouse 71982 Snx10 sorting nexin 10 NM_001127349.1 88.4% 95% (many diffs)
13 mouse 71982 Snx10 sorting nexin 10 NM_028035.4 88.4% 95% (many diffs)
14 mouse 71982 Snx10 sorting nexin 10 XM_006506653.3 88.4% 95% (many diffs)
15 mouse 71982 Snx10 sorting nexin 10 XM_017321753.1 88.4% 95% (many diffs)
16 mouse 71982 Snx10 sorting nexin 10 XM_011241474.1 71.3% 75.1% (many diffs)
17 mouse 71982 Snx10 sorting nexin 10 XM_011241475.1 71.3% 75.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
669
ORF length:
603
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt tccagaacaa cagaaagagg aatttgtaag tgtctgggtt cgagatccta
121ggattcagaa ggaggacttc tggcattctt acattgacta tgagatatgt attcatacta
181atagcatgtg ttttacaatg aaaacaTCCT GTGTACGAAG AAGATATAGA GAATTCGTGT
241GGCTGAGGCA GAGACTCCAA AGTAATGCGT TGCTGGTACA ACTGCCAGAA CTTCCATCTA
301AAAACCTGTT TTTCAACATG AACAATCGCC AGCACGTGGA TCAGCGTCGC CAGGGTCTGG
361AAGATTTCCT CAGAAAAGTC CTACAGAATG CACTTTTGCT TTCATATAGC AGCCTTCACC
421TCTTCTTACA GAGCCATCTG AATTCAGAAG ACATTGAGGC GTGTGTTTCT GGGCAGACTA
481AGTACTCTGT GGAAGAAGCA ATTCACAAGT TTGCCTTAAT GAATAGACGT TTCCCTGAAG
541AAGATGAAGA AGGAAAAAAA GAAAATGATA TAGATTATGA TTCAGAAAGT TCATCCTCTG
601GGCTTGGACA CAGTAGTGAT GACAGCAGTT CACATGGATG TAAAGTAAAT ACAGCTCCGC
661AGGAATCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
721CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
781TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATCCA GAATTGACTT TCCCCTGCAC
841GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)